Iqbal Beenish, Masood Akbar, Lone Mohd Maqbool, Lone Abdul Rashid, Dar Nazir Ahmad
Department of Biochemistry, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Department of Radiation Oncology, SK Institute of Medical Sciences, Srinagar, Jammu and Kashmir, India.
Breast J. 2016 Nov;22(6):673-677. doi: 10.1111/tbj.12668. Epub 2016 Aug 10.
Genetic polymorphisms in metastatic suppressor genes like MKK4 and NME1 are not well studied in breast cancer. Hence, we analyzed the relationship between MKK4 and NME1 polymorphisms and breast cancer risk in Kashmir, India. The different genotypes of NME1 and MKK4 genes were analyzed by polymerase chain reaction and restriction fragment length polymorphism in 130 breast cancer cases and 200 age- and sex-matched controls. Conditional logistic regression models were used to assess the association of various genotypes with breast cancer. In this study, we found an inverse association between MKK4 promoter polymorphism and breast cancer risk. As compared to TT (wild) genotype, individuals with TG (heterozygous) (OR = 0.32; 95% CI = (0.17-0.58) and GG (mutant) (OR = 0.13; CI = 0.04-0.40) genotypes showed decreased risk of breast cancer. When participants were classified on the basis of lymph node involvement, a strong association between NME1 heterozygous genotype (OR = 3.82; CI = (1.54-9.44) and breast cancer was found.
像MKK4和NME1这样的转移抑制基因中的遗传多态性在乳腺癌中尚未得到充分研究。因此，我们分析了印度克什米尔地区MKK4和NME1多态性与乳腺癌风险之间的关系。通过聚合酶链反应和限制性片段长度多态性分析了130例乳腺癌病例和200例年龄及性别匹配的对照中NME1和MKK4基因的不同基因型。使用条件逻辑回归模型评估各种基因型与乳腺癌的关联。在本研究中，我们发现MKK4启动子多态性与乳腺癌风险呈负相关。与TT（野生型）基因型相比，TG（杂合子）（OR = 0.32；95% CI =（0.17 - 0.58））和GG（突变型）（OR = 0.13；CI = 0.04 - 0.40）基因型的个体患乳腺癌的风险降低。当根据淋巴结受累情况对参与者进行分类时，发现NME1杂合子基因型（OR = 3.82；CI =（1.54 - 9.44））与乳腺癌之间存在强关联。
