Hasuike Akira, Sato Shuichi, Udagawa Asami, Ando Kazunari, Arai Yoshinori, Ito Koichi
Division of Applied Oral Sciences, Nihon University School of Dentistry, Tokyo, Japan.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Jan;111(1):e12-20. doi: 10.1016/j.tripleo.2010.09.061.
Low-intensity pulsed ultrasound (LIPUS) is a biophysical intervention in a bone repair process. However, neither the healing events of the flat bones of the skull using LIPUS nor the optimal stimulation settings are fully understood. The aim of this study was to evaluate the bone regenerative effect of LIPUS in rat calvarial flat bone defects by using in vivo microfocus computerized tomography (micro-CT).
The calvarium was exposed, and noncritical-sized 2.7-mm defects were prepared. LIPUS (1.6 MHz, repeating 1.0-kHz pulsation, and 30-mW/cm(2) intensity) was applied for 20 minutes daily. Bone regeneration was evaluated by image analysis using micro-CT and histologic examination.
Within 2 weeks, LIPUS-treated rats demonstrated 7.0% reossification of the original surgical defect, whereas control rats demonstrated 3.6%. At 3 and 4 weeks, a significant difference in the reossification ratio was observed (12.0% vs. 5.8% and 18.1% vs. 9.8%, respectively; P < .05).
LIPUS accelerated bone regeneration of noncritical rat calvarial defects as confirmed by micro-CT.
低强度脉冲超声（LIPUS）是一种用于骨修复过程的生物物理干预手段。然而，使用LIPUS对头盖骨扁骨的愈合情况以及最佳刺激参数尚未完全明确。本研究的目的是通过体内微焦点计算机断层扫描（micro-CT）评估LIPUS对大鼠颅骨扁骨缺损的骨再生作用。
暴露颅骨，制备非临界尺寸的2.7毫米缺损。每天应用LIPUS（1.6兆赫，重复1.0千赫脉冲，强度30毫瓦/平方厘米）20分钟。通过使用micro-CT的图像分析和组织学检查评估骨再生情况。
在2周内，接受LIPUS治疗的大鼠手术缺损处的再骨化率为7.0%，而对照大鼠为3.6%。在3周和4周时，观察到再骨化率存在显著差异（分别为12.0%对5.8%和18.1%对9.8%；P <.05）。
micro-CT证实LIPUS加速了大鼠非临界颅骨缺损的骨再生。
