National Reference Center for Hepatitis B and C in Blood Transfusion, Institut National de la Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France.
J Clin Microbiol. 2011 Mar;49(3):1154-6. doi: 10.1128/JCM.01888-10. Epub 2010 Dec 22.
The new version of the INNO-LiPA HBV genotyping assay (Innogenetics) developed to identify all hepatitis B virus (HBV) genotypes, A to H, has been evaluated in comparison with sequencing of PCR-amplified HBV DNA from 200 samples before or after cloning. The genotyping data obtained with INNO-LiPA were in agreement with those from direct sequencing in the 179 samples characterized by the two methods. INNO-LiPA revealed 28 mixed infections. However, sequencing after molecular cloning confirmed only 15 of them and did not identify any that were of genotype H (n = 9). Our study demonstrates that INNO-LiPA overestimates mixed infections as a result of erroneous genotype H detection.
新的 INNO-LiPA HBV 基因分型检测（Innogenetics）版本旨在鉴定所有乙型肝炎病毒（HBV）基因型，从 A 到 H，已与聚合酶链反应（PCR）扩增的 HBV DNA 克隆前或克隆后的序列比较进行了评估。用 INNO-LiPA 获得的基因分型数据与两种方法鉴定的 179 个样本的直接测序结果一致。INNO-LiPA 显示 28 种混合感染。然而，分子克隆后的测序仅证实了其中的 15 种，并未鉴定出任何基因型 H（n = 9）的感染。我们的研究表明，由于错误的基因型 H 检测，INNO-LiPA 高估了混合感染。
