Garcia Ana Lineth, Parrado Rudy, De Doncker Simonne, Bermudez Hernán, Dujardin Jean-Claude
Laboratory of Molecular Parasitology, Instituut voor Tropische Geneeskunde, Nationalestraat 155, B-2000 Antwerpen, Belgium.
Trans R Soc Trop Med Hyg. 2007 Apr;101(4):368-71. doi: 10.1016/j.trstmh.2006.06.009. Epub 2006 Sep 29.
Species identification is highly relevant for improved prognosis and adequate treatment of American tegumentary leishmaniasis (ATL). PCR-based methods are available for this purpose but should be simplified to improve accessibility. As a first step in this process, this paper describes a simplified protocol for collection of clinical samples. Using samples from 44 Bolivian patients with confirmed ATL, we demonstrated that hsp70 PCR-RFLP on skin scrapings collected with a tooth pick allowed identification of the parasite species with a sensitivity of 95% and specificity of 100%. Our method should greatly facilitate individual patient management and epidemiological surveillance of ATL.
物种鉴定对于改善美洲皮肤利什曼病（ATL）的预后和进行适当治疗至关重要。基于聚合酶链反应（PCR）的方法可用于此目的，但应加以简化以提高其可及性。作为该过程的第一步，本文描述了一种临床样本采集的简化方案。我们使用来自44名确诊为ATL的玻利维亚患者的样本，证明用牙签采集的皮肤刮屑进行热休克蛋白70（hsp70）PCR-限制性片段长度多态性分析（PCR-RFLP）能够鉴定寄生虫物种，灵敏度为95%，特异性为100%。我们的方法应能极大地促进ATL患者的个体管理和流行病学监测。
