将利斯特菌选择性琼脂上的菌落影印接种到血琼脂上，以提高从食品中分离单核细胞增生李斯特菌的效率。
Replica plating of colonies from Listeria-selective agars to blood agar to improve the isolation of Listeria monocytogenes from foods.
作者信息
Cassiday P K, Graves L M, Swaminathan B
机构信息
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia.
出版信息
Appl Environ Microbiol. 1990 Jul;56(7):2274-5. doi: 10.1128/aem.56.7.2274-2275.1990.
Abstract
Bacterial colonies from Listeria-selective agars were replica plated to sheep blood agar to screen for beta-hemolysis. By using the replica plating method to test for the beta-hemolytic characteristic of all the colonies growing on Listeria-selective agars instead of picking 3 to 10 suspected colonies for further testing, we recovered Listeria monocytogenes from 59 of 142 Listeria-selective agar plates which contained colonies of hemolytic and nonhemolytic Listeria species and were negative when tested by conventional colony picks.
摘要
将来自李斯特菌选择性琼脂平板的细菌菌落影印接种到绵羊血琼脂平板上，以筛选β溶血情况。通过使用影印接种法来检测在李斯特菌选择性琼脂平板上生长的所有菌落的β溶血特性，而不是挑选3至10个疑似菌落进行进一步检测，我们从142个李斯特菌选择性琼脂平板中的59个平板上分离出了单核细胞增生李斯特菌，这些平板上既有溶血型也有非溶血型李斯特菌菌落，且通过传统的菌落挑选法检测时呈阴性。
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