将利斯特菌选择性琼脂上的菌落影印接种到血琼脂上，以提高从食品中分离单核细胞增生李斯特菌的效率。
Replica plating of colonies from Listeria-selective agars to blood agar to improve the isolation of Listeria monocytogenes from foods.
作者信息
Cassiday P K, Graves L M, Swaminathan B
机构信息
Division of Bacterial Diseases, Centers for Disease Control, Atlanta, Georgia.
出版信息
Appl Environ Microbiol. 1990 Jul;56(7):2274-5. doi: 10.1128/aem.56.7.2274-2275.1990.
Abstract
Bacterial colonies from Listeria-selective agars were replica plated to sheep blood agar to screen for beta-hemolysis. By using the replica plating method to test for the beta-hemolytic characteristic of all the colonies growing on Listeria-selective agars instead of picking 3 to 10 suspected colonies for further testing, we recovered Listeria monocytogenes from 59 of 142 Listeria-selective agar plates which contained colonies of hemolytic and nonhemolytic Listeria species and were negative when tested by conventional colony picks.
摘要
将来自李斯特菌选择性琼脂平板的细菌菌落影印接种到绵羊血琼脂平板上，以筛选β溶血情况。通过使用影印接种法来检测在李斯特菌选择性琼脂平板上生长的所有菌落的β溶血特性，而不是挑选3至10个疑似菌落进行进一步检测，我们从142个李斯特菌选择性琼脂平板中的59个平板上分离出了单核细胞增生李斯特菌，这些平板上既有溶血型也有非溶血型李斯特菌菌落，且通过传统的菌落挑选法检测时呈阴性。
相似文献
1
Replica plating of colonies from Listeria-selective agars to blood agar to improve the isolation of Listeria monocytogenes from foods.
Appl Environ Microbiol. 1990 Jul;56(7):2274-5. doi: 10.1128/aem.56.7.2274-2275.1990.
2
Evaluation of BBL CHROMagar Listeria agar for the isolation and identification of Listeria monocytogenes from food and environmental samples.
J Microbiol Methods. 2007 Jan;68(1):82-7. doi: 10.1016/j.mimet.2006.06.011. Epub 2006 Aug 23.
3
Evaluation and interlaboratory validation of a selective agar for phosphatidylinositol-specific phospholipase C activity using a chromogenic substrate to detect Listeria monocytogenes from foods.
J Food Prot. 2003 Mar;66(3):441-5. doi: 10.4315/0362-028x-66.3.441.
4
Evaluation of enhanced haemolysis agar for detection of Listeria spp. and L. monocytogenes from production lines of fresh to cold-smoked fish.
J Microbiol Methods. 2001 Aug;46(2):157-63. doi: 10.1016/s0167-7012(01)00264-0.
5
Traditional methods for isolation of Listeria monocytogenes.
Methods Mol Biol. 2014;1157:15-30. doi: 10.1007/978-1-4939-0703-8_2.
6
Comparison of seven plating media for enumeration of Listeria spp.
Appl Environ Microbiol. 1988 Dec;54(12):3003-7. doi: 10.1128/aem.54.12.3003-3007.1988.
7
Optimization of haemolysis in enhanced haemolysis agar (EHA)--a selective medium for the isolation of Listeria monocytogenes.
Lett Appl Microbiol. 1997 May;24(5):421-5. doi: 10.1046/j.1472-765x.1997.00153.x.
8
An improved selective isolation medium for the recovery of Listeria monocytogenes from smoked fish.
Lett Appl Microbiol. 2003;36(4):230-3. doi: 10.1046/j.1472-765x.2003.01298.x.
9
Performance testing of six chromogenic ALOA-type media for the detection of Listeria monocytogenes.
J Appl Microbiol. 2009 Feb;106(2):651-9. doi: 10.1111/j.1365-2672.2008.04039.x.
10
Same-day identification scheme for colonies of Listeria monocytogenes.
Appl Environ Microbiol. 1990 Apr;56(4):1166-8. doi: 10.1128/aem.56.4.1166-1168.1990.
引用本文的文献
1
Use of the CAMP test for identification of Listeria monocytogenes.
Appl Environ Microbiol. 1994 Dec;60(12):4219-25. doi: 10.1128/aem.60.12.4219-4225.1994.
2
Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and nonpathogenic Listeria species.
Appl Environ Microbiol. 1991 Sep;57(9):2666-70. doi: 10.1128/aem.57.9.2666-2670.1991.
本文引用的文献
1
A New Technique for Isolating Listerellae from the Bovine Brain.
J Bacteriol. 1948 Apr;55(4):471-6.
2
Epidemic listeriosis--evidence for transmission by food.
N Engl J Med. 1983 Jan 27;308(4):203-6. doi: 10.1056/NEJM198301273080407.
3
Pasteurized milk as a vehicle of infection in an outbreak of listeriosis.
N Engl J Med. 1985 Feb 14;312(7):404-7. doi: 10.1056/NEJM198502143120704.
4
Monoclonal antibodies which identify a genus-specific Listeria antigen.
Appl Environ Microbiol. 1988 Jun;54(6):1564-9. doi: 10.1128/aem.54.6.1564-1569.1988.
5
Identification and enumeration of beta-hemolytic Listeria monocytogenes in naturally contaminated dairy products.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):673-5.
6
Improved Listeria monocytogenes selective agar.
Appl Environ Microbiol. 1986 Nov;52(5):1215-7. doi: 10.1128/aem.52.5.1215-1217.1986.
7
Liquid and solid selective differential media for the detection and enumeration of L. monocytogenes and other Listeria spp.
Int J Food Microbiol. 1989 Jul;8(4):299-316. doi: 10.1016/0168-1605(89)90001-9.
8
Comparative studies of nucleic acid hybridization assay for Listeria in foods.
J Assoc Off Anal Chem. 1988 May-Jun;71(3):669-73.
Zotero 插件