Beumer R R, te Giffel M C, Cox L J
Laboratory of Food Microbiology and Hygiene, Agricultural University, Wageningen, The Netherlands.
Lett Appl Microbiol. 1997 May;24(5):421-5. doi: 10.1046/j.1472-765x.1997.00153.x.
The presence of Listeria monocytogenes in enrichment media can be masked by faster growth of other Listeria spp. Therefore, enhanced haemolysis agar (EHA) is a good alternative for another isolation media, because the presence of a few L. monocytogenes colonies can be detected in a majority of colonies of other listeriae on the basis of haemolysis. In this study the haemolysis reaction in EHA was optimized. In a collaborative study using reference samples, no significant differences in counts on EHA, Palcam and Oxford agar were shown.
在增菌培养基中,其他李斯特菌属的更快生长可能会掩盖单核细胞增生李斯特菌的存在。因此,增强溶血琼脂(EHA)是另一种分离培养基的良好替代品,因为基于溶血情况,在其他李斯特菌的大多数菌落中可以检测到少数单核细胞增生李斯特菌菌落。在本研究中,对EHA中的溶血反应进行了优化。在一项使用参考样品的协作研究中,EHA、Palcam琼脂和牛津琼脂上的计数没有显示出显著差异。