Department of Urology, The Armed Forces Daejeon Hospital, Daejeon, Korea.
Int Neurourol J. 2010 Oct;14(3):149-56. doi: 10.5213/inj.2010.14.3.149. Epub 2010 Oct 31.
The expression of Nitric oxide Synthase (NOS) and aquaporin (AQP) water channels in rat bladder is recently reported. The aim of this study is to evaluate the expression of inducible NOS (iNOS), aquaporin-3 (AQP-3) in cyclophosphamide (CYP) induced rat bladder.
The 32 Sprague-Dawley rats were divided into cystitis group (n=20) and control group (n=12). In cystitis group, 100mg/kg CYP was injected every second day for 1 week whereas in control group, normal saline was injected. After extracting of the bladder and dividing dome, body and trigone of the bladder, independently H&E staining and immunohistochemical staining for iNOS and AQP-3 were performed. Expressions of iNOS and AQP-3 were analyzed with a confocal laser scanning microscope and an image analyzer.
The expression of iNOS significantly increased in the mucosa, submucosa layer of dome in cystitis group (p<0.05). The expression of AQP-3 significantly increased in the mucosa, submucosa, vessel layer of dome in cystitis group (p<0.05).
These results suggest that inflammatory change activates NOS and AQP-3 expression in the bladder tissue of rats. These may imply that NOS and AQP-3 have a pathophyiological role in the cyclophophamide induced interstitial cystitis. Further study on the NOS and AQP-3 in bladder is needed for clinical application.
最近有报道称，一氧化氮合酶（NOS）和水通道蛋白（AQP）在大鼠膀胱中的表达。本研究旨在评估诱导型 NOS（iNOS）、水通道蛋白-3（AQP-3）在环磷酰胺（CYP）诱导的大鼠膀胱中的表达。
32 只 Sprague-Dawley 大鼠分为膀胱炎组（n=20）和对照组（n=12）。在膀胱炎组中，每两天给 100mg/kg CYP 注射，共 1 周，而在对照组中，注射生理盐水。提取膀胱后，将膀胱的穹隆、体部和三角部分开，分别进行 H&E 染色和 iNOS 和 AQP-3 的免疫组织化学染色。使用共聚焦激光扫描显微镜和图像分析仪分析 iNOS 和 AQP-3 的表达。
膀胱炎组穹隆部黏膜和黏膜下层 iNOS 的表达明显增加（p<0.05）。膀胱炎组穹隆部黏膜、黏膜下层和血管层 AQP-3 的表达明显增加（p<0.05）。
这些结果表明，炎症变化激活了大鼠膀胱组织中 NOS 和 AQP-3 的表达。这可能意味着 NOS 和 AQP-3 在环磷酰胺诱导的间质性膀胱炎中具有病理生理学作用。需要进一步研究膀胱中的 NOS 和 AQP-3，以用于临床应用。
