Qayum A, Gullick W, Clayton R C, Sikora K, Waxman J
Department of Clinical Oncology, Royal Postgraduate Medical School, London, UK.
Br J Cancer. 1990 Jul;62(1):96-9. doi: 10.1038/bjc.1990.236.
We have investigated the possibility of a direct regulatory effect of gonadotrophin releasing hormone (GnRH) analogues on prostatic cancer cell growth. Here we report high affinity binding (Kd = 50 nM) of a GnRH analogue resulting in biphasic growth modulation of the human androgen-sensitive prostatic cancer cell line LNCaP. In contrast, the human androgen-insensitive prostatic cancer cell line DU145 showed low-affinity (Kd = 10 microM) binding without any biological response to the GnRH analogue. A GnRH-specific radioimmunoassay demonstrated GnRH-like immunoreactivity in the concentrated culture medium from both cell lines. Seventy-six human benign and malignant tumours were assayed following surgical resection. Nineteen of 22 (86%) malignant tumours and 49 of 54 (91%) benign tumours, exhibited high affinity GnRH-analogue binding. Fourteen of 19 (74%) malignant tumours and 17 of 49 (35%) benign tumours exhibiting high affinity binding contained GnRH-like immunoreactivity, suggesting that this system may be involved in prostatic epithelial cell growth in vivo.
我们研究了促性腺激素释放激素（GnRH）类似物对前列腺癌细胞生长的直接调节作用的可能性。在此我们报告一种GnRH类似物具有高亲和力结合（解离常数Kd = 50 nM），可导致人雄激素敏感前列腺癌细胞系LNCaP的生长呈双相调节。相比之下，人雄激素不敏感前列腺癌细胞系DU145显示出低亲和力（Kd = 10 microM）结合，对GnRH类似物无任何生物学反应。一种GnRH特异性放射免疫测定法显示，两种细胞系的浓缩培养基中均存在GnRH样免疫反应性。手术切除后对76例人类良性和恶性肿瘤进行了检测。22例恶性肿瘤中的19例（86%）和54例良性肿瘤中的49例（91%）表现出高亲和力的GnRH类似物结合。19例表现出高亲和力结合的恶性肿瘤中的14例（74%）和49例良性肿瘤中的17例（35%）含有GnRH样免疫反应性，提示该系统可能参与体内前列腺上皮细胞的生长。
