[碱性成纤维细胞生长因子对体外培养的牙周膜成纤维细胞β1整合素亚基mRNA表达的影响]
[The effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture].
作者信息
Li Hong-yan, Lin Chong-tao, Li Bo
机构信息
Dept. of Periodontology, Stomatology Hospital of Jilin University, Changchun 130041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Oct;28(5):488-91.
OBJECTIVE
To study the effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture; to discuss the effect of basic fibroblast growth factor in periodontal regeneration.
METHODS
Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth factor (0.1, 1.0, 10.0 ng x mL(-1)) for 24, 48, 72 h respectively, and then mRNA expression of beta1 integrin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction.
RESULTS
Basic fibroblast growth factor enhanced the mRNA expression of beta1 integrin subunit, and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng x mL(-1) at 24, 48, 72 h respectively; the mRNA expression of beta1 integrin subunit at 72 h was higher than that at 24, 48 h.
CONCLUSION
Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts' adhesion and may be one of important factors which participate in the periodontal regeneration.
目的
研究碱性成纤维细胞生长因子对体外培养的牙周膜成纤维细胞β1整合素亚单位mRNA表达的影响；探讨碱性成纤维细胞生长因子在牙周组织再生中的作用。
方法
体外培养人牙周膜成纤维细胞，分别用碱性成纤维细胞生长因子（0.1、1.0、10.0 ng·mL⁻¹）刺激24、48、72 h，然后采用荧光定量实时逆转录聚合酶链反应检测β1整合素亚单位的mRNA表达。
结果
碱性成纤维细胞生长因子可增强β1整合素亚单位的mRNA表达，在24、48、72 h时，碱性成纤维细胞生长因子浓度为1.0 ng·mL⁻¹时作用最佳；β1整合素亚单位的mRNA表达在72 h时高于24、48 h时。
结论
碱性成纤维细胞生长因子可增强人牙周膜成纤维细胞的黏附能力，可能是参与牙周组织再生的重要因子之一。
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