State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuihan 430072, China.
J Environ Sci (China). 2010;22(8):1225-31. doi: 10.1016/s1001-0742(09)60242-4.
Phytoremediation of triazophos (O,O-diethyl-O-(1-phenyl-1,2,4-triazole-3-base) sulfur phosphate, TAP) pollution by Canna indica Lim. in a hydroponic system has been well studied, whereas the microbial mechanism on TAP degradation is still unknown. The variation in microbial community compositions was investigated by analyzing phospholipid fatty acids (PLFAs) profiles in microbes under TAP exposure. The TAP exposure resulted in an increase in proportions of fatty acid 16:0 and decrease in fatty acid 18:2omega9,12c, indicating that TAP may stimulate the reproduction of microorganisms and inhibit the growth of fungi to some degree. Significant correlation was found between the ratio of fungi to bacteria and TAP removal (r2 = 0.840, p < 0.01). In addition, the microbial community in the phytoremediation system with C. indica was dominated by Gram negative bacteria, which possibly contributed to the degradation of TAP. These results indicated that TAP might induce the colonization of bacteria in the hydroponic system planted with C. indica, and lead to a discrimination of microbial community, which might be one of the mechanisms on TAP dissipation in phytoremediation system.
水培系统中美人蕉（Canna indica Lim.）对三唑磷（O,O-二乙基-O-（1-苯基-1,2,4-三唑-3-基）硫代磷酸酯，TAP）污染的植物修复已有大量研究，但 TAP 降解的微生物机制仍不清楚。通过分析 TAP 暴露下微生物中磷脂脂肪酸（PLFA）谱，研究了微生物群落组成的变化。TAP 暴露导致 16:0 脂肪酸比例增加，18:2omega9,12c 脂肪酸减少，表明 TAP 可能在一定程度上刺激微生物的繁殖，抑制真菌的生长。真菌与细菌的比例与 TAP 去除率之间存在显著相关性（r2 = 0.840，p < 0.01）。此外，美人蕉植物修复系统中的微生物群落主要由革兰氏阴性菌组成，这可能有助于 TAP 的降解。这些结果表明，TAP 可能诱导水培系统中细菌的定植，并导致微生物群落的区分，这可能是植物修复系统中 TAP 消散的机制之一。
