Zhejiang Provincial Key Laboratory of Subtropic Soil and Plant Nutrition, College of Environmental and Resource Sciences, Zhejiang University (Huajiachi Campus), Hangzhou 310029, China.
J Environ Sci (China). 2010;22(8):1253-60. doi: 10.1016/s1001-0742(09)60246-1.
Lime application is a conventional technology to control acidification in tea orchard soils. We investigated the effect of lime application on soil microbial community diversity in the soils of three tea orchards, wasteland and forest. The BIOLOG data showed that both the average well color development of all carbon sources and the functional diversity index increased with the liming rate in the tea orchards and the forest, but decreased in the wasteland. The phospholipid fatty acid (PLFA) analysis showed that the structural diversity index of soil microbial community increased with the liming rate in all the tea orchards, the wasteland and the forest. Lime application also increased the soil-bacterial PLFA content in all the soils. Soil fungal and actinomycete PLFAs in the tea orchards showed an increasing trend from 0 to 3.2 g CaCO3/kg application and then a decreasing trend from 3.2 to 6.4 g CaCO3/kg application. The principal component analysis of BIOLOG and PLFA data suggested that lime application had a significant effect on soil microbial community structure, and land use had a greater effect on soil microbial community structure compared to lime application.
石灰应用是控制茶园土壤酸化的传统技术。我们研究了石灰应用对三个茶园、荒地和林地土壤微生物群落多样性的影响。BIOLOG 数据表明，茶园和林地中所有碳源的平均好氧颜色发展和功能多样性指数都随施石灰率的增加而增加，而荒地则相反。磷脂脂肪酸（PLFA）分析表明，茶园、荒地和林地土壤微生物群落的结构多样性指数随施石灰率的增加而增加。石灰应用还增加了所有土壤中的土壤细菌 PLFA 含量。茶园土壤真菌和放线菌 PLFA 从 0 到 3.2 g CaCO3/kg 应用呈增加趋势，然后从 3.2 到 6.4 g CaCO3/kg 应用呈减少趋势。BIOLOG 和 PLFA 数据的主成分分析表明，石灰应用对土壤微生物群落结构有显著影响，与石灰应用相比，土地利用对土壤微生物群落结构的影响更大。
