Rojek Sebastian, Kłys Małgorzata, Rzepecka-Woźniak Ewa, Konopka Tomasz
Z Katedry i Zakładu Medycyny Sadowej UJ CM, Kraków.
Arch Med Sadowej Kryminol. 2010 Jan-Mar;60(1):12-7.
The study represents an attempt at employing segmental hair analysis in complex poisonings with xenobiotic mixtures of heroine - cocaine - amphetamines in the context of the cause of death as a consequence of complex interaction mechanisms which occurred prior to death. Two cases of complex poisonings: heroine - cocaine and heroine - cocaine - amphetamines were analyzed and documented with macro- and microscopic examinations and complex toxicological examinations, including the analysis of classic biological material, i.e. samples of selective blood, and alternative material, i.e. hair samples. Determinations of opioids, cocaine and its metabolite and amphetamines in the hair biological matrix were performed using high performance liquid chromatography--atmospheric pressure chemical ionization--tandem mass spectrometry (HPLC-APCI-MS-MS). Segmental hair analysis of the investigated cases indicated a prolonged intake of similar psychoactive substances and a developed adaptation of the addicted to interaction mechanisms, which, however, led gradually to multiorgan anatomopathological changes, and in consequence to death.
该研究试图在因死亡前发生的复杂相互作用机制导致的死亡原因背景下，对海洛因 - 可卡因 - 苯丙胺类外源性混合物复杂中毒病例采用分段毛发分析。分析并记录了两例复杂中毒病例：海洛因 - 可卡因中毒和海洛因 - 可卡因 - 苯丙胺类中毒，采用了宏观和微观检查以及复杂的毒理学检查，包括对经典生物材料（即选择性血液样本）和替代材料（即毛发样本）的分析。使用高效液相色谱 - 大气压化学电离 - 串联质谱法（HPLC - APCI - MS - MS）对毛发生物基质中的阿片类药物、可卡因及其代谢物和苯丙胺类进行测定。对所调查病例的分段毛发分析表明，类似精神活性物质的摄入时间延长，成瘾者对相互作用机制产生了适应性变化，然而，这逐渐导致多器官解剖病理学改变，最终导致死亡。
