[I组代谢型谷氨酸受体反义寡核苷酸对培养的小鼠大脑皮质神经元谷氨酸神经毒性的保护作用]
[The protective effects of group I mGluRs antisense oligonucleotides against neurotoxicity of sodium glutamate on cultured mouse cerebral cortical neurons].
作者信息
Dong Li-ping, Han Ming, Yuan Fang
机构信息
Beijing Neurosurgical Institute, Beijing 100050, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Nov;23(4):438-41.
AIM
To investigate the protective effect of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides on impairment of cultured mouse cerebral cortical neurons induced by sodium glutamate (Glu).
METHODS
Neuron damage induced by Glu as well as the action of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides were measured by determining the leakage of lactate dehydrogenase (LDH) from neurons. Immunocytochemistry method was used to detect the expression of anti-mGluRl a and anti-mGluR5. Morphological changes of primary cortical neurons were observed by phase contrast microscope.
RESULTS
Following the exposure of the cells to 0.1 mmol x L(-1) Glu for 15 min, LDH leakage from neurons increased. mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides(6 or 8 micromol x L(-1)) as well as 50 micromol x L(-1) LY367385 reduced the LDH leakage. mGluR1alpha and mGluR5 immunopositive cells showed in cultured neurons.
CONCLUSION
The protective effects of mGluR1 antisense oligonucleotides and mGluR5 antisense oligonucleotides on neurons damaged by Glu may relate to antagonizing mGluR1a or mGItlR5.
目的
研究代谢型谷氨酸受体1（mGluR1）反义寡核苷酸和代谢型谷氨酸受体5（mGluR5）反义寡核苷酸对谷氨酸（Glu）诱导的培养小鼠大脑皮质神经元损伤的保护作用。
方法
通过测定神经元中乳酸脱氢酶（LDH）的漏出量来检测Glu诱导的神经元损伤以及mGluR1反义寡核苷酸和mGluR5反义寡核苷酸的作用。采用免疫细胞化学方法检测抗mGluR1α和抗mGluR5的表达。用相差显微镜观察原代皮质神经元的形态变化。
结果
细胞暴露于0.1 mmol·L⁻¹ Glu 15分钟后，神经元的LDH漏出量增加。mGluR1反义寡核苷酸和mGluR5反义寡核苷酸（6或8 μmol·L⁻¹）以及50 μmol·L⁻¹ LY367385可减少LDH漏出。在培养的神经元中可见mGluR1α和mGluR5免疫阳性细胞。
结论
mGluR1反义寡核苷酸和mGluR5反义寡核苷酸对Glu损伤神经元的保护作用可能与拮抗mGluR1α或mGluR5有关。
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