酶联免疫吸附测定中抗哇巴因蛋黄抗体（IgY）与兔抗体（IgG）的比较
[Comparison between anti-ouabain egg yolk(IgY) and rabbit antibody(IgG) in enzyme-linked immunosorbent assay].
作者信息
Zhang Ming-juan, Yang Jun, Ge Heng, Qiang Lei, Duan Zong-ming, Wang Cong-xia, Wang Rong, Lu Zhuo-rern
机构信息
Department of Cardiology, Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Nov;23(4):505-8.
AIM
To improve specificity and accuracy of endogenous ouabain measurement assay.
METHODS
Anti-ouabain polyclonal antibody egg yolk (IgY) and anti-ouabain rabbit antibody (IgG) were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay (ELISA) were compared.
RESULTS
The ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation(CV) was 2.03%, and the inter-assay CV was 2.34% respectively. In contrast, IgG were 2.83% and 3.29%. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs. 5.95%, 3.20% vs. 5.20% of crossreaction with cedilanid and digoxin.
CONCLUSION
The specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.
目的
提高内源性哇巴因测量分析的特异性和准确性。
方法
分别制备抗哇巴因多克隆抗体蛋黄（IgY）和抗哇巴因兔抗体（IgG）。在两种抗体存在的情况下，比较酶联免疫吸附测定（ELISA）的特异性和准确性。
结果
在存在IgY的情况下，ELISA的平均批内变异系数（CV）为2.03%，批间CV分别为2.34%。相比之下，IgG分别为2.83%和3.29%。氢化可的松和地塞米松未观察到明显干扰。与西地兰和地高辛的交叉反应分别为3.45%对5.95%，3.20%对5.20%。
结论
使用IgY的ELISA的特异性和准确性比IgG更好。
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