Hatta H, Tsuda K, Akachi S, Kim M, Yamamoto T
Central Research Laboratories, Taiyo Kagaku Co., Ltd., Mie, Japan.
Biosci Biotechnol Biochem. 1993 Mar;57(3):450-4. doi: 10.1271/bbb.57.450.
Productivity and some properties of anti-Human Rotavirus (HRV) hen egg yolk antibody (IgY) were compared with those of anti-HRV rabbit serum antibody (IgG). The hens immunized with HRV (Wa strain, serotype 1 and Mo strain, serotype 3) were found to continuously to lay eggs without any change in the egg laying rate and the yolk of the eggs laid over a year showed a high level of neutralization titer against HRV. The production of anti-HRV IgY by a hen (one year) was at least 15 times (anti-Wa) and 120 times (anti-Mo) more effective than those by an immunized rabbit in the neutralization titer of the antibodies. The stability of anti-HRV IgY at temperature above 70 degrees C and low pH 2-3 was less than that of anti-HRV rabbit IgG. The temperature corresponding to the maximum of denaturation endotherm (Tmax) of IgY was 73.9 degrees C while that of rabbit IgG was 77.0 degrees C in the analysis by differential scanning calorimetry. This discrepancy in heat and acidic pH stability found between the two antibodies as discussed with regard to their protein structures.
将抗人轮状病毒（HRV）的鸡卵黄抗体（IgY）与抗HRV兔血清抗体（IgG）的生产效率及某些特性进行了比较。用HRV（1型Wa株和3型Mo株）免疫的母鸡被发现能持续产蛋，产蛋率没有任何变化，并且在一年中产下的鸡蛋蛋黄对HRV显示出高水平的中和效价。在抗体中和效价方面，一只母鸡（一年）产生的抗HRV IgY比免疫兔产生的抗HRV IgY至少有效15倍（抗Wa）和120倍（抗Mo）。抗HRV IgY在70℃以上温度和低pH 2 - 3条件下的稳定性低于抗HRV兔IgG。在差示扫描量热法分析中，IgY变性吸热最大值对应的温度（Tmax）为73.9℃，而兔IgG为77.0℃。正如根据两种抗体的蛋白质结构所讨论的那样，发现这两种抗体在热稳定性和酸性pH稳定性方面存在差异。
