Yang Sheng-Mei, Du Ji-Zeng
College of Biosciences and Biotechnology, Yangzhou University, Yangzhou 225009, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Nov;21(4):419-22.
To investigate the response of hypothalamic corticotropin releasing factor (CRF) and arginine vasopressin (AVP) of adrenalectomized neonatal rats to hypoxia.
The hypoxia was simulated in hypobaric cabin. The amount of arginine vasopressin (AVP) and corticotropin releasing factor (CRF) was assayed by RIA method.
When neonatal rats exposed to acute hypoxia (simulated altitude of 5 000 m and 7 000 m, 24 h), their hypothalamic CRF was not changed in 3 d and 7 d rats, lower than control in 14 d, 21 d and 28 d rats, while hypothalamic AVP had no change in 3 d rats, was lower than control in 14 d and higher in 7 d, 21 d and 28 d rats. The responsive pattern of those two neuropeptides to acute hypoxia changed with the growth of rats. Adrenalectomy reduced the amount of hypothalamic CRF and AVP in 14 d, 21 d and 28 d rats, except AVP in 28 d rats. When adrenalectomized neonates exposed to hypoxia, there was no further change in CRF and AVE, means adrenalectomy not only removed the negative regulation of corticosteroid on CRF and AVE, but also inhibited the normal development of CRF and AVP neurons and thus, their responsive ability to hypoxia.
研究新生大鼠肾上腺切除术后下丘脑促肾上腺皮质激素释放因子（CRF）和精氨酸加压素（AVP）对低氧的反应。
在低压舱内模拟低氧环境。采用放射免疫分析法测定精氨酸加压素（AVP）和促肾上腺皮质激素释放因子（CRF）的含量。
新生大鼠暴露于急性低氧环境（模拟海拔5000米和7000米，24小时）时，3日龄和7日龄大鼠下丘脑CRF无变化，14日龄、21日龄和28日龄大鼠下丘脑CRF低于对照组；3日龄大鼠下丘脑AVP无变化，14日龄大鼠下丘脑AVP低于对照组，7日龄、21日龄和28日龄大鼠下丘脑AVP高于对照组。这两种神经肽对急性低氧的反应模式随大鼠生长而变化。肾上腺切除术降低了14日龄、21日龄和28日龄大鼠下丘脑CRF和AVP的含量，但28日龄大鼠的AVP除外。肾上腺切除的新生大鼠暴露于低氧环境时，CRF和AVP无进一步变化，这意味着肾上腺切除术不仅消除了皮质类固醇对CRF和AVP的负调节作用，还抑制了CRF和AVP神经元的正常发育，从而使其对低氧的反应能力受到抑制。
