Rodrigues Silvia Victor, Acharya Anirudh B, Bhadbhade Smruti, Thakur Srinath L
Department of Periodontics, SDM College of Dental Sciences & Hospital, Karnataka, India.
Oral Health Prev Dent. 2010;8(4):389-94.
Hyaluronan, commonly known as hyaluronic acid, has been shown to have anti-inflammatory action, bacteriostatic effect and antioxidant properties, thus making its use as a long-term anti-plaque and anti-gingivitis agent an appealing proposition. The aim of the present study was to evaluate the efficacy of 0.025% hyaluronan-containing mouthwash in comparison with 0.2% chlorhexidine and a water-based mouthwash and also to evaluate its antibacterial efficacy on isolated strains of periodontopathogens.
Forty-five volunteers in a hospital setting were recruited. A single-blinded, parallel design, randomised controlled trial was carried out and the 4-day plaque re-growth model was used to study the efficacy of the three mouthwashes. Microbiological and clinical evaluation was performed by culturing and using dental indices, respectively. The three mouthwashes used in the present study were commercially available 0.025% sodium hyaluronate, 0.2% chlorhexidine and a water-based rinse (negative control). Effects of the three mouthwashes were tested on the growth of isolated strains of Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa) and Prevotella intermedia (Pi).
In vitro, hyaluronan had a distinct effect on the growth of Aa and Pi with no effect on the growth of Pg. In vivo, the differences between the individual rinse solutions and the water-based solution showed significantly less plaque regrowth with respect to both chlorhexidine (P = 0.033) and hyaluronan (P = 0.045) when compared to the negative control. The difference between chlorhexidine and hyaluronan was not statistically significant (P = 0.69).
Hyaluronan (0.025%)-containing mouthwash was comparable to chlorhexidine (0.2%) in inhibiting plaque growth in vivo, and it significantly reduced the growth of Aa and Pi in vitro
透明质酸，通常称为玻尿酸，已被证明具有抗炎作用、抑菌效果和抗氧化特性，因此将其用作长期抗牙菌斑和抗牙龈炎药物是一个有吸引力的提议。本研究的目的是评估含0.025%透明质酸的漱口水与0.2%氯己定和水性漱口水相比的疗效，并评估其对分离出的牙周病原体菌株的抗菌效果。
招募了医院环境中的45名志愿者。进行了一项单盲、平行设计、随机对照试验，并使用4天菌斑再生长模型来研究三种漱口水的疗效。分别通过培养和使用牙科指数进行微生物学和临床评估。本研究中使用的三种漱口水是市售的0.025%透明质酸钠、0.2%氯己定和水性漱口水（阴性对照）。测试了三种漱口水对牙龈卟啉单胞菌（Pg）、伴放线聚集杆菌（Aa）和中间普氏菌（Pi）分离菌株生长的影响。
在体外，透明质酸对Aa和Pi的生长有明显影响，对Pg的生长无影响。在体内，与阴性对照相比，各漱口水溶液与水性溶液之间的差异显示，氯己定（P = 0.033）和透明质酸（P = 0.045）的菌斑再生长明显较少。氯己定和透明质酸之间的差异无统计学意义（P = 0.69）。
含0.025%透明质酸的漱口水在体内抑制菌斑生长方面与0.2%氯己定相当，并且在体外显著降低了Aa和Pi的生长
