Mishra Mahendra Narain, Bedi Varinder Singh
Department of Pathology, Command Hospital, Mumbai, Maharashtra, India.
Sao Paulo Med J. 2010;128(5):263-7. doi: 10.1590/s1516-31802010000500004.
venous thrombosis occurs as a result of interaction of genetic and acquired factors including activated protein C resistance (APC-R), fibrinogen levels, antithrombin, protein C, protein S, lupus anticoagulants and anticardiolipin antibodies. This study was aimed at determining the prevalence of these common thrombophilia markers in Asian Indians with primary venous thrombosis.
this was a cross-sectional study carried out in Mumbai.
samples from 78 patients with a confirmed diagnosis of venous thrombosis and 50 controls were tested. Semi-quantitative estimation (functional assays) of protein C, protein S and antithrombin was performed. Quantitative estimation of fibrinogen was done using the Clauss method. Lupus anticoagulants were screened using lupus-sensitive activated partial thromboplastin time and β2-glycoprotein-I dependent anticardiolipin antibodies were estimated by ELISA. APC-R was measured using a clotting-based method with factor V deficient plasma and Crotalus viridis venom. Statistical analysis was performed using Epi-info (version 6).
the popliteal vein was the most commonly involved site. Forty-four samples (56%) gave abnormal results. The commonest were elevated fibrinogen and APC-R (17.9% each), followed by low protein S (16.6%).
this study confirms the literature findings that fibrinogen level estimation and screening for APC-R are important for the work-up on venous thrombosis patients since these, singly or in combination, may lead to a primary thrombotic episode. The frequency of the other thrombophilia markers was higher among the patients than among the controls, but without statistically significant difference.
静脉血栓形成是遗传因素与后天因素相互作用的结果，这些因素包括活化蛋白C抵抗（APC-R）、纤维蛋白原水平、抗凝血酶、蛋白C、蛋白S、狼疮抗凝物和抗心磷脂抗体。本研究旨在确定这些常见血栓形成倾向标志物在亚洲印度裔原发性静脉血栓患者中的患病率。
这是一项在孟买开展的横断面研究。
对78例确诊为静脉血栓的患者和50例对照者的样本进行检测。对蛋白C、蛋白S和抗凝血酶进行半定量评估（功能测定）。使用克劳斯法对纤维蛋白原进行定量评估。使用狼疮敏感的活化部分凝血活酶时间筛查狼疮抗凝物，通过酶联免疫吸附测定法评估β2-糖蛋白-I依赖性抗心磷脂抗体。使用基于凝血的方法，采用缺乏因子V的血浆和绿曼巴蛇毒来测量APC-R。使用Epi-info（6版）进行统计分析。
腘静脉是最常受累的部位。44份样本（56%）结果异常。最常见的是纤维蛋白原升高和APC-R升高（各占17.9%），其次是蛋白S降低（16.6%）。
本研究证实了文献中的发现，即纤维蛋白原水平评估和APC-R筛查对于静脉血栓患者的检查很重要，因为这些因素单独或联合作用可能导致原发性血栓形成事件。其他血栓形成倾向标志物在患者中的频率高于对照组，但无统计学显著差异。
