National Reference Laboratory of Enteroviruses, Department of Virology, National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
J Med Virol. 2011 Feb;83(2):348-56. doi: 10.1002/jmv.21919.
Annually 20-70% of all hospital admissions and 20% of fatal diarrhea cases among children less than 5 years of age occur due to severe rotavirus diarrhea. Universal immunization is the major strategy aimed at controlling rotavirus infection. The main objective of the present study was to elucidate the evolutionary relationships of the most common rotavirus strains co-circulating in Bulgaria. The sequence and phylogenetic analysis revealed strain diversity and circulation of different rotavirus variants belonging to a single genotype. A mutated G4P[8] strain with the insertion of an asparagine residue in position 76; G2, G9, and G1 variants with amino acid substitutions in the antigenic regions A, B, and/or C were all identified in this study in the absence of an immunization program. Rotavirus strain surveillance in both the pre- and post-vaccine eras is of increasing importance in order to assess the effectiveness of the rotavirus vaccines for protection against disease associated with a diverse population of rotavirus strains.
每年有 20%至 70%的住院病例和 20%5 岁以下儿童的致死性腹泻病例是由严重轮状病毒腹泻引起的。普遍免疫是旨在控制轮状病毒感染的主要策略。本研究的主要目的是阐明保加利亚流行的最常见轮状病毒株的进化关系。序列和系统发育分析显示，不同的轮状病毒变异株存在多样性和循环，它们属于单一基因型。在没有免疫计划的情况下，本研究中发现了一种突变的 G4P[8]株，其在位置 76 插入了一个天冬酰胺残基；G2、G9 和 G1 变体在抗原区域 A、B 和/或 C 中存在氨基酸取代。在疫苗接种前后，轮状病毒株的监测对于评估轮状病毒疫苗对与不同人群的轮状病毒株相关疾病的保护效果越来越重要。
