Departamento de Virología, Laboratorio de Gastroenteritis Virales, INEI-ANLIS Dr Carlos G Malbrán, Buenos Aires, Argentina.
Infect Genet Evol. 2009 Dec;9(6):1225-31. doi: 10.1016/j.meegid.2009.07.002. Epub 2009 Jul 23.
Group A rotaviruses are the most frequently detected viral agents associated with diarrhea in infants and children worldwide. It has been estimated that every year almost 120,000 cases of diarrhea associated with rotavirus occur in children under 5 years old in Argentina. In this work, we present the rotavirus strain diversity detected during the first 2 years of the National Surveillance Network for Diarrheas implemented by the Ministry of Health in Argentina. During 2006 and 2007 a total of 464 rotavirus positive samples were G and P genotyped. The predominant genotype combination was G9P[8] (54.1%), followed by G2P[4] (26.5%) and G4P[8] (4.3%). Of note is that four samples were found possessing the G3 genotype, and two the genotype combination G4P[6]. The phylogenetic analysis of the VP7 gene grouped the Argentinean G9 and G3 strains within the lineages currently circulating in humans worldwide, i.e. lineages III and Ia respectively; however, the sequence and phylogenetic analyses of the VP7, NSP4 and the VP8* fragment from the Argentinean G4P[6] strains suggest a porcine origin. In agreement with this, the phylogenetic tree of the VP7 gene from G4 strains suggests the presence of at least two porcine lineages currently circulating in the Americas. In addition, the inclusion of new sequences available in public databases and the sequences reported in this work allowed us to describe new lineages and sublineages within the G4 and P[6] genotypes.
A 组轮状病毒是全球范围内与婴儿和儿童腹泻相关的最常见的病毒病原体。据估计，在阿根廷，每年有近 120000 例 5 岁以下儿童因轮状病毒腹泻。在这项工作中，我们展示了在阿根廷卫生部实施的全国腹泻监测网络的前 2 年中检测到的轮状病毒株多样性。在 2006 年和 2007 年，总共对 464 个轮状病毒阳性样本进行了 G 和 P 基因型分析。占主导地位的基因型组合是 G9P[8]（54.1%），其次是 G2P[4]（26.5%）和 G4P[8]（4.3%）。值得注意的是，有四个样本被发现具有 G3 基因型，两个样本具有 G4P[6]基因型组合。VP7 基因的系统进化分析将阿根廷的 G9 和 G3 株系归入目前在全球人类中流行的谱系，即分别为 III 和 Ia 谱系；然而，对来自阿根廷 G4P[6]株系的 VP7、NSP4 和 VP8*片段的序列和系统进化分析表明，这些株系来源于猪。这与 VP7 基因的系统进化树一致，表明至少有两个猪谱系目前正在美洲流行。此外，包括公共数据库中可用的新序列和本工作中报告的序列，使我们能够在 G4 和 P[6]基因型内描述新的谱系和亚谱系。
