开发了一个 URA5 整合盒，用于 Candida guilliermondii ATCC 6260 菌株的基因敲除。
Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain.
机构信息
Biomolécules et Biotechnologies Végétales, Faculté de Pharmacie, Université François Rabelais, Tours, France.
出版信息
J Microbiol Methods. 2011 Feb;84(2):355-8. doi: 10.1016/j.mimet.2010.12.013. Epub 2010 Dec 21.
PMID:21182877
Abstract
We designed an efficient transformation system for Candida guilliermondii based on a ura5 ATCC 6260 derived recipient strain and a URA5 recyclable selection marker. This "URA5 blaster" disruption system represents a powerful tool to study the function of a large pallet of genes in this yeast of clinical and biotechnological interest.
摘要
我们设计了一个基于源自 ura5 ATCC 6260 的 Candida guilliermondii 受体菌株和可回收 URA5 选择标记的高效转化系统。这个“URA5 爆破器”敲除系统是研究该具有临床和生物技术应用价值的酵母中大量基因功能的有力工具。
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