AMPA 受体亚基和 TARPs 在成年大鼠伏隔核突触和非突触膜中的分布。
Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens.
机构信息
Department of Neuroscience, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, IL 60064 3095, United States.
出版信息
Neurosci Lett. 2011 Mar 3;490(3):180-4. doi: 10.1016/j.neulet.2010.12.036. Epub 2010 Dec 21.
Abstract
We characterized the distribution of AMPA receptor (AMPAR) subunits and the transmembrane AMPA receptor regulatory proteins (TARPs) γ-2 and γ-4 in adult rat nucleus accumbens (NAc) using a method that separates plasma membranes into synaptic membrane-enriched and extrasynaptic membrane-enriched fractions. We also measured GluA1 phosphorylated at serine 845 (pS845 GluA1) and serine 831 (pS831 GluA1). GluA1-3 protein levels and pS831 GluA1/total GluA1 were higher in synaptic membranes. However, pS845 GluA1/total GluA1 was higher in extrasynaptic membranes, consistent with a role for S845 phosphorylation in GluA1 insertion at extrasynaptic sites. Homeric GluA1 receptors were detected in extrasynaptic membranes, consistent with evidence for extrasynaptic Ca(2+)-permeable AMPARs in other systems. The TARP γ-2 was enriched in synaptic membranes, whereas γ-4 was mainly found in extrasynaptic membranes, suggesting distinct roles for these proteins in the NAc. These experiments provide fundamental information that will aid in the interpretation of studies on AMPAR-related plasticity in the NAc.
摘要
我们使用一种将质膜分离为富含突触膜和 extrasynaptic 膜的方法，研究了成年大鼠伏隔核（NAc）中 AMPA 受体（AMPAR）亚基和跨膜 AMPA 受体调节蛋白（TARPs）γ-2 和 γ-4 的分布。我们还测量了 GluA1 在丝氨酸 845（pS845 GluA1）和丝氨酸 831（pS831 GluA1）处的磷酸化。GluA1-3 蛋白水平和 pS831 GluA1/总 GluA1 在突触膜中较高。然而，pS845 GluA1/总 GluA1 在 extrasynaptic 膜中较高，与 S845 磷酸化在 extrasynaptic 部位 GluA1 插入中的作用一致。 Homeric GluA1 受体在 extrasynaptic 膜中被检测到，与其他系统中存在 extrasynaptic Ca(2+)-permeable AMPAR 的证据一致。TARP γ-2 在突触膜中富集，而 γ-4 主要在 extrasynaptic 膜中发现，表明这些蛋白在 NAc 中具有不同的作用。这些实验提供了基本信息，将有助于解释 NAc 中与 AMPAR 相关的可塑性研究。
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