Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University,Cardiff, United Kingdom.
J Cataract Refract Surg. 2011 Jan;37(1):166-72. doi: 10.1016/j.jcrs.2010.07.028.
To evaluate 3 approaches, both cellular and acellular, to improve the healing of laser in situ keratomileusis flaps in bovine corneas.
School of Optometry and Vision Sciences and Cardiff Institute of Tissue Engineering and Repair, Cardiff University, Cardiff, United Kingdom.
Experimental study.
Laser in situ keratomileusis-like flaps were created in bovine corneas, and the flap bed was treated with tumor necrosis factor-α, interleukin-1α, Fas ligand, transforming growth factor-β(1), or activated stromal fibroblasts. In separate experiments, flaps were created and repositioned. The corneas were then crosslinked using ultraviolet-A (UVA) light. All samples were then placed in organ culture for up to 4 weeks. Untreated samples acted as controls.
All treatments increased the adherence of the stromal flap. This was achieved at the expense of corneal clarity except in the case of crosslinking (CXL). In this case, the flap adhesion force immediately increased while the cornea remained clear. The force then decreased gradually during organ culture, although it remained at twice the level of the control corneas after 3 weeks in culture.
The results suggest that riboflavin-UVA CXL is a hopeful approach for increasing the adherence strength of corneal flaps while keeping the cornea clear. Further studies are necessary to confirm the durability of the strengthening effect and to exclude serious late complications.
No author has a financial or proprietary interest in any material or method mentioned.
评估 3 种方法，包括细胞和去细胞方法，以改善牛眼角膜中的激光原位角膜磨镶术瓣的愈合。
英国卡迪夫大学视光与视觉科学学院和卡迪夫组织工程与修复研究所。
实验研究。
在牛眼角膜上制作类似激光原位角膜磨镶术的瓣，并用肿瘤坏死因子-α、白细胞介素-1α、Fas 配体、转化生长因子-β(1)或激活的基质成纤维细胞处理瓣床。在单独的实验中，制作并重新定位瓣。然后使用紫外线-A（UVA）光对角膜进行交联。所有样本随后都被放置在器官培养中长达 4 周。未处理的样本作为对照。
所有处理方法都增加了基质瓣的粘附力。这是通过牺牲角膜清晰度来实现的，除了交联（CXL）的情况。在这种情况下，瓣的粘附力立即增加，而角膜仍然保持清晰。然后在器官培养过程中逐渐下降，但在培养 3 周后，其仍保持在对照角膜的两倍水平。
结果表明，核黄素-UVA CXL 是一种有希望的方法，可以在保持角膜清晰的同时增加角膜瓣的粘附强度。需要进一步的研究来确认强化效果的耐久性，并排除严重的迟发性并发症。
没有作者在提到的任何材料或方法中有经济或所有权利益。
