School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK.
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
Microbiology (Reading). 2011 Apr;157(Pt 4):1115-1122. doi: 10.1099/mic.0.045807-0. Epub 2010 Dec 23.
Manganese has an important yet undefined role in the virulence of many bacterial pathogens. In this study we confirm that a null mutation in Yersinia pseudotuberculosis mntH reduces intracellular manganese accumulation. An mntH mutant was susceptible to killing by reactive oxygen species when grown under manganese-limited conditions. The mntH mutant was defective in survival and growth in macrophages expressing functional Nramp1, but in macrophages deficient in Nramp the bacteria were able to survive and replicate. In Galleria mellonella, the mntH mutant was attenuated. Taken together, these data suggest a role for manganese in Y. pseudotuberculosis during macrophage intracellular survival, protecting the bacteria from the antimicrobial products released during the respiratory burst.
锰在许多细菌病原体的毒力中具有重要但尚未确定的作用。在这项研究中，我们证实，假结核耶尔森氏菌 mntH 的缺失突变会降低细胞内锰的积累。在锰限制条件下生长时，mntH 突变体易被活性氧杀死。mntH 突变体在表达功能正常的 Nramp1 的巨噬细胞中的存活和生长能力受损，但在 Nramp 缺乏的巨噬细胞中，细菌能够存活和复制。在大蜡螟中，mntH 突变体的毒力减弱。综上所述，这些数据表明锰在假结核耶尔森氏菌的巨噬细胞内存活过程中发挥作用，保护细菌免受呼吸爆发期间释放的抗菌产物的影响。
