AGO 蛋白的结构、功能与调控。
Form, function, and regulation of ARGONAUTE proteins.
机构信息
Institut Jean-Pierre Bourgin, Unité Mixte de Recherche 1318, Institut National de la Recherche Agronomique, 78026 Versailles Cedex, France.
出版信息
Plant Cell. 2010 Dec;22(12):3879-89. doi: 10.1105/tpc.110.080671. Epub 2010 Dec 23.
Abstract
Both transcriptional (TGS) and posttranscriptional gene silencing (PTGS) are conserved eukaryotic gene regulatory mechanisms, integral for taming exogenous (viruses and bacteria) or endogenous (repetitive elements and transposons) invasive nucleic acids to minimize their impact on genome integrity and function. TGS and PTGS also are essential for controlling the expression of protein coding genes throughout development or in response to environmental stimuli. In plants and animals, at least one member of the conserved ARGONAUTE (AGO) protein family comprises the catalytic engine of the silencing complex, which is guided by sequence-specific small RNA to cognate RNA. In this review, we present general features of plant and animal AGO proteins and detail our knowledge on the 10 Arabidopsis thaliana AGOs.
摘要
转录基因沉默（TGS）和转录后基因沉默（PTGS）是保守的真核基因调控机制，对于驯化外源（病毒和细菌）或内源（重复元件和转座子）入侵核酸以最小化它们对基因组完整性和功能的影响至关重要。TGS 和 PTGS 对于控制蛋白质编码基因在整个发育过程中的表达或响应环境刺激也是必不可少的。在植物和动物中，保守的 ARGONAUTE（AGO）蛋白家族的至少一个成员构成沉默复合物的催化引擎，该引擎由序列特异性小 RNA 引导至同源 RNA。在这篇综述中，我们介绍了植物和动物 AGO 蛋白的一般特征，并详细介绍了我们对 10 个拟南芥 AGO 的了解。
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