Yao Jia, Qiao Zesen, Jiang Ziming, Zhao Xueru, You Ziyang, Zhang Wenzhe, Feng Jiancan, Gong Chenrui, Li Jidong
College of Forestry, Henan Agricultural University, Zhengzhou 450046, China.
College of Horticulture, Henan Agricultural University, Zhengzhou 450046, China.
Microorganisms. 2025 Mar 14;13(3):658. doi: 10.3390/microorganisms13030658.
The cultivation of jujube () in China is threatened by jujube witches' broom (JWB) disease, a devastating infectious disease associated with JWB phytoplasma (' Phytoplasma ziziphi'). In many plants, proteins in the Argonaute (AGO) family, as main components of the RNA-induced silencing complex (RISC), play important roles in RNA silencing and pathogen resistance. The jujube telomere-to-telomere genome was searched by BLAST using Arabidopsis AGOs as probes. A total of nine jujube AGO gene members were identified, with each containing the conserved N-terminal, PZA, and PIWI domains. Phylogenetic analysis revealed that the nine jujube AGOs scattered into all three AGO clades. Expression patterns of the genes were analyzed in response to phytoplasma in transcriptome data and by qRT-PCR. The jujube-phytoplasma interaction altered the expression of jujube genes. and were up-regulated in the majority of the eight sampling periods subjected to qRT-PCR analysis. In the transcriptome data, and were also up-regulated during the key stages 37 and 39 weeks after grafting (WAG) with phytoplasma-infected material. These two jujube genes may play important roles in response to JWB phytoplasma infection.
在中国，枣树（）的种植受到枣疯病（JWB）的威胁，枣疯病是一种与枣疯病植原体（‘Phytoplasma ziziphi’）相关的毁灭性传染病。在许多植物中，作为RNA诱导沉默复合体（RISC）主要成分的AGO（Argonaute）家族蛋白在RNA沉默和病原体抗性中发挥重要作用。以拟南芥AGO蛋白为探针，通过BLAST搜索枣的端粒到端粒基因组。共鉴定出9个枣AGO基因成员，每个成员都包含保守的N端、PZA和PIWI结构域。系统发育分析表明，9个枣AGO蛋白分散在所有三个AGO进化枝中。利用转录组数据和qRT-PCR分析了这些基因响应植原体的表达模式。枣与植原体的相互作用改变了枣基因的表达。在qRT-PCR分析的8个采样期的大多数时期，和均上调。在转录组数据中，在嫁接感染植原体材料后的关键阶段第37周和第39周（WAG），和也上调。这两个枣基因可能在响应枣疯病植原体感染中发挥重要作用。
