Gogoi Beauty, Das Indukalpa, Gogoi Madhurjya, Charingia Dipika, Bandyopadhyay Tanoy, Borah Debajit
Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, 786004 India.
Department of Biotechnology, Assam Royal Global University, Guwahati, 781035 India.
3 Biotech. 2022 Mar;12(3):68. doi: 10.1007/s13205-022-03133-2. Epub 2022 Feb 12.
Production of biosurfactant by a novel indigenous isolate strain DU13 and its role in bioremediation of petroleum hydrocarbon is reported. The identity of the isolate was confirmed by 16S rDNA gene sequencing analysis (Genbank accession: MK177190). The biosurfactant produced by the isolate could reduce the surface tension of petroleum supplemented medium by 46% just after 7 days of treatment. The emulsification index ( ) of the surfactant was found 37, 35, and 20%, respectively, against used motor oil, diesel, and kerosene. The FTIR spectrum of the crude biosurfactant showed the presence of υ stretch, υ υ stretch and υ bonding. The isolated strain could degrade 26% of TPH content of used motor oil in liquid culture. Whereas, ex situ pilot-scale field trial demonstrated very high bioremediation potential of the isolate in terms of germination rate of and seeds and plant growth just after 20 days of treatment.
报道了一种新型本土分离菌株DU13产生生物表面活性剂及其在石油烃生物修复中的作用。通过16S rDNA基因测序分析（Genbank登录号：MK177190）确认了该分离株的身份。该分离株产生的生物表面活性剂在处理7天后就能使添加石油的培养基表面张力降低46%。该表面活性剂对废机油、柴油和煤油的乳化指数分别为37%、35%和20%。粗生物表面活性剂的FTIR光谱显示存在υ伸缩振动、υ υ伸缩振动和υ键。该分离菌株在液体培养中能降解废机油中26%的总石油烃含量。而异地中试规模田间试验表明，处理20天后，该分离株在种子发芽率和植物生长方面具有很高的生物修复潜力。
