Abelev G I, Karamova E R, Bragina E G, Andreeva N E, Kruglova G V
Laboratory of Immunochemistry, All-Union Cancer Research Center, Academy of Medical Sciences, Moscow, USSR.
Int J Cancer. 1990 Sep 15;46(3):351-5. doi: 10.1002/ijc.2910460302.
We suggest that countercurrent isotachophoresis performed on cellulose acetate membranes (ITP-CAM) should be used for detecting trace amounts of Bence-Jones protein (BJP) in urine of patients with chronic lymphocytic leukemia (CLL), non-Hodgkin's lymphoma (NHL) and related diseases. ITP-CAM allows simultaneous concentration and electrophoretic separation of proteins present in highly diluted solutions, as well as easy immunological detection of separated substances. BJP was found in 24 out of 42 patients with CLL, 33 of 56 with NHL and 3 of 3 with Waldenström macroglobulinemia. Twenty-three patients were followed during the course of chemo- or radiotherapy. In 19 cases the BJP findings correlated well with clinical status. In no case of partial or complete clinical response did BJP completely disappear from the urine.
我们建议，应使用在醋酸纤维素膜上进行的逆流等速电泳（ITP-CAM）来检测慢性淋巴细胞白血病（CLL）、非霍奇金淋巴瘤（NHL）及相关疾病患者尿液中的微量本-周蛋白（BJP）。ITP-CAM可对高度稀释溶液中的蛋白质同时进行浓缩和电泳分离，还便于对分离出的物质进行免疫检测。在42例CLL患者中有24例检测到BJP，56例NHL患者中有33例，3例瓦尔登斯特伦巨球蛋白血症患者中有3例检测到BJP。23例患者在化疗或放疗过程中接受了随访。在19例患者中，BJP检测结果与临床状况高度相关。在部分或完全临床缓解的病例中，BJP从未在尿液中完全消失。
