Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1HH, UK.
Methods. 2011 Apr;53(4):366-71. doi: 10.1016/j.ymeth.2010.12.022. Epub 2010 Dec 23.
Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.
转座子是遗传筛选中一种很有吸引力的系统，因为它们在分子水平上是可操作的，并且产生所需表型的被破坏的基因座很容易被定位。本文考虑了 piggyBac 转座子系统在补充现有的哺乳动物筛选策略中的特点，包括 Sleeping Beauty 转座子系统。我们还描述了为正向和反向遗传筛选而开发的 piggyBac 资源的设计，以及我们在这些实验中使用的方案。
