Department of Anatomy, University of Hong Kong, Hong Kong, China.
Nanomedicine. 2011 Jun;7(3):351-9. doi: 10.1016/j.nano.2010.12.001. Epub 2010 Dec 23.
To speed up the process of central nervous system (CNS) recovery after injury, the need for real-time measurement of axon regeneration in vivo is essential to assess the extent of injury, as well as the optimal timing and delivery of therapeutics and rehabilitation. It was necessary to develop a chronic animal model with an in vivo measurement technique to provide a real-time monitoring and feedback system. Using the framework of the 4 P's of CNS regeneration (Preserve, Permit, Promote and Plasticity) as a guide, combined with noninvasive manganese-enhanced magnetic resonance imaging (MEMRI), we show a successful chronic injury model to measure CNS regeneration, combined with an in vivo measurement system to provide real-time feedback during every stage of the regeneration process. We also show that a chronic optic tract (OT) lesion is able to heal, and axons are able to regenerate, when treated with a self-assembling nanofiber peptide scaffold (SAPNS).
The authors of this study demonstrate the development of a chronic injury model to measure CNS regeneration, combined with an in vivo measurement system to provide real-time feedback during every stage of the regeneration process. In addition, they determined that chronic optic tract lesions are able to heal with axonal regeneration when treated with a self-assembling nanofiber peptide scaffold (SAPNS).
为了加快中枢神经系统 (CNS) 损伤后的恢复进程，有必要实时测量体内轴突再生，以评估损伤程度以及治疗和康复的最佳时机和方式。因此，有必要开发一种具有体内测量技术的慢性动物模型，以提供实时监测和反馈系统。我们以 CNS 再生的 4P 原则（Preserve、Permit、Promote 和 Plasticity）为指导，结合非侵入性锰增强磁共振成像 (MEMRI)，展示了一种成功的慢性损伤模型，用于测量 CNS 再生，并结合体内测量系统，在再生过程的每个阶段提供实时反馈。我们还表明，当使用自组装纳米纤维肽支架 (SAPNS) 治疗时，慢性视束 (OT) 损伤能够愈合，轴突能够再生。
本研究的作者展示了一种慢性损伤模型的开发，用于测量中枢神经系统再生，并结合体内测量系统，在再生过程的每个阶段提供实时反馈。此外，他们确定，当用自组装纳米纤维肽支架 (SAPNS) 治疗时，慢性视束损伤能够愈合并伴有轴突再生。
