Denyer S P, Davies M C, Evans J A, Finch R G, Smith D G, Wilcox M H, Williams P
Department of Pharmaceutical Sciences, University of Nottingham, United Kingdom.
J Clin Microbiol. 1990 Aug;28(8):1813-7. doi: 10.1128/jcm.28.8.1813-1817.1990.
Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the surface chemistries of cells grown in the two atmospheres, as determined by X-ray photoelectron spectroscopy and changes in the cell wall protein profile. The growth atmosphere also influenced the adherence potential toward polystyrene and silicone in a proportion of strains examined. Thus, gaseous conditions can profoundly influence the nature of the staphylococcal surface, and this should be considered in any in vitro study of in vivo behavior.
从持续性非卧床腹膜透析相关性腹膜炎患者身上获取的凝固酶阴性葡萄球菌，在含有5%二氧化碳的空气环境（反映新鲜使用的透析液中的二氧化碳张力）的营养肉汤中培养。通过X射线光电子能谱和细胞壁蛋白谱的变化确定，在两种环境中生长的细胞表面化学性质存在显著差异。生长环境也影响了所检测的一部分菌株对聚苯乙烯和硅胶的黏附潜力。因此，气体条件可深刻影响葡萄球菌表面的性质，在任何关于体内行为的体外研究中都应考虑这一点。
