Department of Applied Bioscience, CHA University, Seoul 135-081, Republic of Korea.
Department of Life Science & Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Nov;61(Pt 11):2729-2733. doi: 10.1099/ijs.0.029173-0. Epub 2010 Dec 24.
A Gram-staining-negative, strictly aerobic bacterium, designated strain G3(T), was isolated from a tidal flat of the Taean coast in South Korea. Cells were moderately halotolerant and non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain G3(T) was observed between 15 and 40 °C (optimum 30 °C) and between pH 5.5 and 9.0 (optimum pH 6.5-7.5). Strain G3(T) contained Q-8 as the predominant lipoquinone and iso-C(15 : 0), iso-C(17 : 1)ω9c, iso-C(16 : 0) and iso-C(11 : 0) as the major fatty acids. The G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain G3(T) formed a tight phylogenetic lineage with Luteimonas mephitis B1953/27.1(T) within the genus Luteimonas and was most closely related to L. mephitis B1953/27.1(T) with 98.0 % 16S rRNA gene sequence similarity. The DNA-DNA relatedness between strain G3(T) and L. mephitis B1953/27.1(T) was 35.2 ± 3.3 %. On the basis of chemotaxonomic data and molecular properties, strain G3(T) represents a novel species of the genus Luteimonas, for which the name Luteimonas lutimaris sp. nov. is proposed. The type strain is G3(T) (= KACC 14929(T) = JCM 16916(T)).
一株革兰氏阴性、严格需氧的细菌，被命名为 G3(T) 菌株，从韩国泰安海岸的潮滩中分离得到。细胞中度耐盐，为不运动的杆状，呈过氧化氢酶和氧化酶阳性反应。G3(T) 菌株的生长温度为 15 到 40°C（最适温度 30°C），pH 值为 5.5 到 9.0（最适 pH 值为 6.5-7.5）。该菌株含有 Q-8 作为主要的脂醌，以及异-C(15:0)、iso-C(17:1)ω9c、iso-C(16:0) 和 iso-C(11:0) 作为主要脂肪酸。基因组 DNA 的 G+C 含量为 69.6mol%。基于 16S rRNA 基因序列的系统发育分析表明，G3(T) 菌株与 Luteimonas mephitis B1953/27.1(T) 一起形成了 Luteimonas 属内一个紧密的系统发育分支，与 L. mephitis B1953/27.1(T) 的 16S rRNA 基因序列相似性最高，为 98.0%。G3(T) 菌株与 L. mephitis B1953/27.1(T) 之间的 DNA-DNA 同源性为 35.2±3.3%。基于化学分类数据和分子特性，G3(T) 菌株代表了 Luteimonas 属的一个新种，命名为 Luteimonas lutimaris sp. nov.。其模式菌株为 G3(T)（=KACC 14929(T)=JCM 16916(T)）。
