State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
Langmuir. 2011 Feb 1;27(3):874-7. doi: 10.1021/la103799e. Epub 2010 Dec 27.
In this Letter, we demonstrate that chemical oxidation polymerization of o-phenylenediamine (OPD) by potassium bichromate at room temperature results in the formation of submicrometer-scale poly(o-phenylenediamine) (POPD) colloids. Such colloids can absorb and quench dye-labeled single-stranded DNA (ssDNA) very effectively. In the presence of a target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the POPD surface, leading to recovery of dye fluorescence. With the use of an oligonucleotide (OND) sequence associated with human immunodeficiency virus (HIV) as a model system, we demonstrate the proof of concept that POPD colloid-quenched fluorescent OND can be used as a probe for fluorescence-enhanced nucleic acid detection with selectivity down to single-base mismatch.
在这封信件中，我们证明了邻苯二胺（OPD）在室温下通过重铬酸钾的化学氧化聚合反应生成亚微米级别的聚邻苯二胺（POPD）胶体。这种胶体可以非常有效地吸收和猝灭染料标记的单链 DNA（ssDNA）。在存在靶标的情况下，发生杂交事件，产生从 POPD 表面脱离的双链 DNA（dsDNA），从而恢复染料荧光。使用与人类免疫缺陷病毒（HIV）相关的寡核苷酸（OND）序列作为模型系统，我们证明了概念验证，即 POPD 胶体猝灭荧光 OND 可作为荧光增强核酸检测的探针，其选择性可低至单个碱基错配。
