Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, 04103 Leipzig, Germany.
Virus Res. 2011 Feb;155(2):514-9. doi: 10.1016/j.virusres.2010.12.009. Epub 2010 Dec 25.
Chimpanzee polyomavirus (ChPyV) was originally detected in the faeces of a captive chimpanzee by random screening using broad-spectrum PCR. Its pathogenicity and the distribution among chimpanzees are unknown so far. Here, the major capsid protein VP1 of ChPyV was expressed in yeast cells. Virus-like particles (VLPs) with a diameter of approximately 45nm were demonstrated although the efficiency of VLP formation was low as compared to monkey polyomavirus SV40-VLPs. The ChPyV-VLP preparation did not agglutinate human erythrocytes. Low cross-reactions between ChPyV- and SV40-VLP-specific sera were detected by immunoblotting, but not by ELISA. Testing of 163 sera derived from captive and wild-caught healthy chimpanzees using an ELISA based on the ChPyV-VLPs resulted in 11.7% positive results, ranging from 0% to 56% in different groups. The VLPs may be used in future to assess the distribution of ChPyV infections among other animal species and humans.
黑猩猩多瘤病毒（ChPyV）最初是通过广谱 PCR 随机筛查从圈养黑猩猩的粪便中检测到的。其致病性和在黑猩猩中的分布尚不清楚。在此，利用酵母细胞表达了 ChPyV 的主要衣壳蛋白 VP1。尽管与猴多瘤病毒 SV40-VLPs 相比，VLP 的形成效率较低，但仍能证明其形成了直径约为 45nm 的病毒样颗粒（VLPs）。ChPyV-VLP 制剂不能凝集人红细胞。通过免疫印迹检测到 ChPyV-VLP 和 SV40-VLP 特异性血清之间的低交叉反应，但通过 ELISA 检测不到。使用基于 ChPyV-VLPs 的 ELISA 检测来自圈养和野生捕获的健康黑猩猩的 163 份血清，结果显示阳性率为 11.7%，不同组的阳性率范围为 0%至 56%。VLPs 将来可用于评估 ChPyV 感染在其他动物物种和人类中的分布。
