West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Accra 00233, Ghana.
Department of Medical Laboratory Sciences, University of Ghana, Accra 00233, Ghana.
Exp Biol Med (Maywood). 2023 Jul;248(14):1221-1228. doi: 10.1177/15353702231181355. Epub 2023 Jul 7.
Gastric cancer (GC) prevalence is on the increase in Ghana, and Epstein-Barr virus (EBV) is one of the factors that have been implicated in the etiology of the cancer. It is therefore important to know the contribution of EBV genotype and strains that are associated with GC. In this study, we aimed at genotyping EBV and determining predominant strains in GC biopsies in Ghanaian patients. Genomic DNA was extracted from 55 GC biopsies (cases) and 63 normal gastric tissues (controls) were amplified by polymerase chain reaction (PCR) using specific primers for EBV detection and genotyping followed by PCR fragments sequencing. Epstein-Barr virus positivity were 67.3% and 49.2% in the GC and normal biopsies, respectively. Both cases and controls had the Mediterranean + strain of EBV. The predominant genotype of the virus in the GC cases was genotype-1 (75.7%) compared to 66.7% of genotype-2 among the control group. Infection was associated with GC in the study population (OR = 2.11, = 0.014, 95% CI: 1.19 - 3.75), and EBV genotype-1 significantly increased the risk of GC (OR = 5.88, < 0.0001, 95% CI: 3.18-10.88). The mean EBV load in the cases (3.507 ± 0.574) was significantly higher than in the controls (2.256 ± 0.756) ( < 0.0001). We conclude that EBV, especially Mediterranean + genotype-1, was the predominant strain in GC biopsies and GC type or progression is independent of the viral load.
在加纳，胃癌（GC）的患病率呈上升趋势，而 Epstein-Barr 病毒（EBV）是导致癌症的因素之一。因此，了解与 GC 相关的 EBV 基因型和毒株的贡献非常重要。在这项研究中，我们旨在对 EBV 进行基因分型，并确定加纳 GC 活检中的优势株。从 55 份 GC 活检（病例）和 63 份正常胃组织（对照）中提取基因组 DNA，使用 EBV 检测和基因分型的特异性引物通过聚合酶链反应（PCR）扩增，然后对 PCR 片段进行测序。GC 和正常活检中的 EBV 阳性率分别为 67.3%和 49.2%。病例和对照组均存在 EBV 的地中海+株。GC 病例中病毒的主要基因型为基因型-1（75.7%），而对照组中基因型-2 的比例为 66.7%。在研究人群中，感染与 GC 相关（OR = 2.11， = 0.014，95%CI：1.19 - 3.75），并且 EBV 基因型-1 显著增加了 GC 的风险（OR = 5.88， < 0.0001，95%CI：3.18-10.88）。病例组的 EBV 载量平均值（3.507 ± 0.574）明显高于对照组（2.256 ± 0.756）（ < 0.0001）。我们得出结论，EBV，特别是地中海+基因型-1，是 GC 活检中的主要菌株，GC 类型或进展与病毒载量无关。
