采用七种体外扩增检测方法分析口腔和口咽鳞状细胞癌中的 HPV 基因型分布。

HPV genotype distribution in oral and oropharyngeal squamous cell carcinoma using seven in vitro amplification assays.

机构信息

epartments of 1Pathology, Prevention Research Center, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

出版信息

Anticancer Res. 2010 Dec;30(12):5099-104.

PMID:21187496

Abstract

BACKGROUND

Molecular and epidemiologic evidence indicates that human papillomavirus (HPV) is involved in the etiology of oral and oropharyngeal squamous cell carcinomas (SCCs). HPV(+) tumors appear to be clinically distinct from HPV(-) tumors, conferring improved survival outcomes for patients. Determination of the HPV status of tumors may assist in patient risk-stratification and ultimately guide optimum treatment. The primary aim of this study was to examine the distribution of HPV in oral and oropharyngeal SCCs as assessed using seven different in vitro amplification assays. The secondary aim was to correlate the distribution of HPV in tumors with clinical and demographic patient data.

MATERIALS AND METHODS

Sixty-eight invasive oral/oropharyngeal SCCs were tested for HPV using four laboratory-developed PCR assays for HPV16 or 18 and three commercial tests, INNO-LiPA® HPV Genotyping Extra (Innogenetics), Linear Array® HPV Genotyping Test (Roche Diagnostics), and Invader® HPV16/18 ASRs (Hologic Corp.).

RESULTS

Consensus results between tests revealed that 71.9% of tumors were HPV(+), primarily with HPV16 (63.2%). Other genotypes were uncommon and generally occurred coincidently with HPV16. HPV-positivity was significantly higher in oropharyngeal tumors (76.9%), particularly of the tonsils (91.7%), versus oral cavity tumors (20.0%). HPV(+) tumors occurred in younger patients (average 54.4 years versus 61.1 years) and were significantly associated with lower histological differentiation (poorly, 100.0%; moderately, 65.6%; well-differentiated, 42.9%).

CONCLUSION

A high rate of HPV-positivity, especially involving HPV16, occurred in oropharyngeal tumors, with a lower rate in oral cavity SCCs; however, solitary infections with HPV18, 33 or 45 in a minority of cases signified the potential oncogenicity of these additional genotypes and the likely need to screen for these less common genotypes in clinical specimens.

摘要

背景

分子和流行病学证据表明，人乳头瘤病毒（HPV）与口腔和口咽鳞状细胞癌（SCC）的病因有关。HPV（+）肿瘤在临床上与 HPV（-）肿瘤明显不同，为患者带来更好的生存结果。确定肿瘤的 HPV 状态可能有助于患者风险分层，并最终指导最佳治疗。本研究的主要目的是使用七种不同的体外扩增检测方法检查口腔和口咽 SCC 中 HPV 的分布。次要目的是将肿瘤中 HPV 的分布与临床和人口统计学患者数据相关联。

材料和方法

使用四种针对 HPV16 或 18 的实验室开发的 PCR 检测方法和三种商业检测方法（Innogenetics 的 INNO-LiPA® HPV 基因分型 Extra、Roche Diagnostics 的 Linear Array® HPV 基因分型测试和 Hologic Corp. 的 Invader® HPV16/18 ASRs），对 68 例侵袭性口腔/口咽 SCC 进行 HPV 检测。

结果

测试之间的共识结果显示，71.9%的肿瘤为 HPV（+），主要为 HPV16（63.2%）。其他基因型很少见，通常与 HPV16 同时发生。HPV 阳性率在口咽肿瘤（76.9%），特别是扁桃体（91.7%）中明显高于口腔肿瘤（20.0%）。HPV（+）肿瘤发生在年轻患者（平均 54.4 岁 vs. 61.1 岁），且与组织学分化程度较低显著相关（低分化，100.0%；中分化，65.6%；高分化，42.9%）。