比较两种线印迹分析方法在口腔和口咽鳞状细胞癌中定义 HPV 基因型的应用。

Comparison of 2 line blot assays for defining HPV genotypes in oral and oropharyngeal squamous cell carcinomas.

机构信息

Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

出版信息

Diagn Microbiol Infect Dis. 2011 Jun;70(2):240-5. doi: 10.1016/j.diagmicrobio.2011.01.011. Epub 2011 Mar 9.

DOI:10.1016/j.diagmicrobio.2011.01.011

Abstract

Patients with invasive oral and oropharyngeal squamous cell carcinomas infected with human papillomaviruses (HPV) demonstrate improved survival. HPV detection in tumors may assist in risk stratification of patients and in guiding optimum treatment. Two reverse line blot assays [Linear Array (LA) and INNO-LiPA (LiPA)] were evaluated for detection of HPV genotypes in paraffin-embedded biopsies. Overall, 82.4% of 131 biopsies were HPV+ by LiPA versus 61.1% by LA (κ = 0.32). Completely concordant results were observed in 52.7% of cases: 18 negative and 51 with exactly the same genotype(s). An additional 13 cases had partial agreement. These 82 completely or partially concordant cases revealed a high rate of HPV positivity (78.0%), primarily involving HPV16 (90.6%). HPV+ tumors occurred preferentially in the oropharynx, especially tonsils, with trends for male patients and poor differentiation. Significant differences in these associations were found when LA and LiPA results were analyzed independently. No relationships were found between tumor HPV status and tobacco or alcohol use.

摘要

HPV 感染的侵袭性口腔和口咽鳞状细胞癌患者的生存率提高。肿瘤中 HPV 的检测可辅助患者的危险分层，并指导最佳治疗。评估了两种反向线印迹检测法[线性阵列（LA）和 INNO-LiPA（LiPA）]用于检测石蜡包埋活检中的 HPV 基因型。总体而言，LiPA 检测到的 131 个活检中有 82.4%为 HPV+，而 LA 为 61.1%（κ=0.32）。52.7%的病例结果完全一致：18 例为阴性，51 例具有完全相同的基因型。另有 13 例存在部分一致。这 82 例完全或部分一致的病例显示出 HPV 阳性率高（78.0%），主要涉及 HPV16（90.6%）。HPV+肿瘤更常发生于口咽，尤其是扁桃体，男性患者和低分化趋势。当分别分析 LA 和 LiPA 的结果时，发现这些关联存在显著差异。未发现肿瘤 HPV 状态与烟草或酒精使用之间存在关系。