Kim Jong Rhan, Seok Seung Hyeok, Kim Dong Jae, Baek Min-Won, Na Yi-Rang, Han Ju-Hee, Kim Tae-Hyun, Park Jae-Hak, Turner Patricia V, Chung Doo Hyun, Kang Byeong-Cheol
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Korea.
J Vet Med Sci. 2011 May;73(5):687-91. doi: 10.1292/jvms.10-0226. Epub 2010 Dec 22.
Currently, murine noroviruses (MNV) are the most prevalent viral pathogens identified in laboratory animal facilities. While several reports exist concerning the prevalence of MNV in North American research facilities, very few reports are available for other parts of the world, including Korea. This study evaluated the prevalence of MNV infection in 745 murine sera collected from 15 animal facilities in Korea by enzyme linked immunosorbent assay (ELISA). Positive cases were subcategorized by murine strain/genetics, housing environments and animal sources. In summary, 6.6% of inbred/outbred mice purchased from commercial vendors were seropositive, 9.6% of in-house colonies were seropositive and 27.0% of genetically modified mice (GMM) were seropositive. Partial gene amplification of fecal isolates from infected animals showed that they were homologous (100%) with MNV-4.
目前，鼠诺如病毒（MNV）是实验动物设施中鉴定出的最普遍的病毒病原体。虽然有几份关于北美研究设施中MNV流行情况的报告，但世界其他地区，包括韩国，相关报告却很少。本研究通过酶联免疫吸附测定（ELISA）评估了从韩国15个动物设施收集的745份鼠血清中MNV感染的流行情况。阳性病例按鼠种系/遗传学、饲养环境和动物来源进行了分类。总之，从商业供应商购买的近交/远交小鼠中有6.6%血清呈阳性，内部繁殖群体中有9.6%血清呈阳性，转基因小鼠（GMM）中有27.0%血清呈阳性。对感染动物粪便分离株的部分基因扩增表明，它们与MNV-4同源（100%）。
