Departamento de Inmunología, Facultad de Medicina, UASLP, Ave. V. Carranza 2405, 78210, San Luis Potosí, SLP, Mexico.
J Clin Immunol. 2011 Jun;31(3):369-78. doi: 10.1007/s10875-010-9496-0. Epub 2010 Dec 29.
Human leukocyte antigen (HLA)-G is a class I non-classical HLA molecule with an important regulatory role on the immune response. The possible role of this molecule in the pathogenesis of SLE has not been explored. In this work, we evaluated the expression and function of HLA-G in SLE patients. We studied 37 SLE patients as well as 25 healthy donors. Peripheral blood monocytes and in vitro-generated dendritic cells (DCs) were analyzed for HLA-G expression by flow cytometry. We found that monocytes from SLE patients as well as mature CD83+ DCs showed a diminished expression of HLA-G compared with healthy controls. In addition, monocytes from SLE patients showed a diminished induction of HLA-G expression in response to stimulation with IL-10. Furthermore, functional assays showed that these monocytes pre-treated with IFN-γ exhibited a diminished capability to inhibit the proliferation of autologous lymphocytes. Finally, lymphocytes from SLE patients tended to display a lower acquisition of HLA-G (by trogocytosis) from autologous monocytes compared to controls. Our results might have implications for the immune abnormalities observed in patients with SLE.
人类白细胞抗原（HLA）-G 是一种 I 类非经典 HLA 分子，对免疫反应具有重要的调节作用。该分子在系统性红斑狼疮（SLE）发病机制中的可能作用尚未得到探索。在这项工作中，我们评估了 HLA-G 在 SLE 患者中的表达和功能。我们研究了 37 名 SLE 患者和 25 名健康供体。通过流式细胞术分析外周血单核细胞和体外生成的树突状细胞（DC）中 HLA-G 的表达。我们发现与健康对照组相比，SLE 患者的单核细胞和成熟的 CD83+DC 显示 HLA-G 表达减少。此外，SLE 患者的单核细胞在受到 IL-10 刺激时 HLA-G 表达的诱导减少。此外，功能测定表明，这些经 IFN-γ预处理的单核细胞抑制自身淋巴细胞增殖的能力降低。最后，与对照组相比，SLE 患者的淋巴细胞从自身单核细胞中摄取 HLA-G（通过 trogocytosis）的能力较低。我们的结果可能对 SLE 患者中观察到的免疫异常具有重要意义。
