School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150, Kubang Kerian, Kelantan, Malaysia.
Neurochem Res. 2011 Mar;36(3):533-9. doi: 10.1007/s11064-010-0375-0. Epub 2010 Dec 25.
Downstream Regulatory Element Antagonist Modulator (DREAM) protein modulates pain by regulating prodynorphin gene transcription. Therefore, we investigate the changes of mRNA and DREAM protein in relation to the mRNA and prodynorphin protein expression on the ipsilateral side of the rat spinal cord after formalin injection (acute pain model). DREAM like immunoreactivity (DLI) was not significantly different between C and F groups. However, we detected the upregulation of mean relative DREAM protein level in the nuclear but not in the cytoplasmic extract in the F group. These effects were consistent with the upregulation of the relative DREAM mRNA level. Prodynorphin like immunoreactivity (PLI) expression increased but the relative prodynorphin mRNA level remained unchanged. In conclusion, we suggest that upregulation of DREAM mRNA and protein expression in the nuclear compartment probably has functional consequences other than just the repression of prodynorphin gene. It is likely that these mechanisms are important in the modulation of pain.
下游调节元件拮抗剂调制器 (DREAM) 蛋白通过调节前强啡肽原基因转录来调节疼痛。因此，我们研究了福尔马林注射后（急性疼痛模型）大鼠脊髓同侧 mRNA 和 DREAM 蛋白与 mRNA 和前强啡肽原蛋白表达的变化。C 组和 F 组之间的 DREAM 样免疫反应性 (DLI) 没有显著差异。然而，我们在 F 组中检测到核提取物中平均相对 DREAM 蛋白水平的上调，但在细胞质提取物中没有上调。这些效应与相对 DREAM mRNA 水平的上调一致。前强啡肽样免疫反应性 (PLI) 表达增加，但相对前强啡肽原 mRNA 水平保持不变。总之，我们认为核区 DREAM mRNA 和蛋白表达的上调可能除了抑制前强啡肽原基因外，还有其他功能后果。这些机制很可能在疼痛调节中很重要。
