Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Neurotox Res. 2018 Feb;33(2):402-411. doi: 10.1007/s12640-017-9806-x. Epub 2017 Sep 21.
Neurogenesis is influenced by various external factors such as enriched environments. Some researchers had postulated that neurogenesis has contributed to the hippocampal learning and memory. This project was designed to observe the effect of Delta-9-tetrahydrocannabinol (∆-THC) in cognitive performance that influenced by the neurogenesis. Different doses of ∆-THC were used for observing the neurogenesis mechanism occurs in the hippocampus of rats. The brains were stained with antibodies, namely BrdU, glial fibrillary acidic protein (GFAP), nestin, doublecortin (DCX) and class III β-tubulin (TuJ-1). The cognitive test was used novel-object discrimination test (NOD) while the proteins involved, DCX and brain-derived neurotrophic factor (BDNF), were measured. Throughout this study, ∆-THC enhanced the markers involved in all stages of neurogenesis mechanism. Simultaneously, the cognitive behaviour of rat also showed improvement in learning and memory functions observed in behavioural test and molecular perspective. Administration of ∆-THC was observed to enhance the neurogenesis in the brain, especially in hippocampus thus improved the cognitive function of rats.
神经发生受多种外部因素的影响，如丰富的环境。一些研究人员假设神经发生有助于海马体的学习和记忆。本项目旨在观察神经发生对认知表现的影响，使用不同剂量的∆-THC 观察大鼠海马体中的神经发生机制。用抗体（BrdU、胶质纤维酸性蛋白（GFAP）、巢蛋白、双皮质素（DCX）和 III 类β-微管蛋白（TuJ-1））对大脑进行染色。认知测试使用新物体识别测试（NOD），同时测量涉及的蛋白质，即 DCX 和脑源性神经营养因子（BDNF）。在整个研究过程中，∆-THC 增强了神经发生机制所有阶段涉及的标志物。同时，在行为测试和分子水平上，观察到大鼠的认知行为在学习和记忆功能方面也有所改善。∆-THC 的给药观察到增强了大脑，特别是海马体中的神经发生，从而改善了大鼠的认知功能。
