Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
Curr Microbiol. 2011 Apr;62(4):1267-73. doi: 10.1007/s00284-010-9852-6. Epub 2010 Dec 25.
Glycine betaine (GB) could be used by Arthrobacter globiformis cells as a sole carbon source. The cells took up this molecule in the low as well as in the high salinity medium. Addition of GB to the mineral medium with high salt concentration revealed that GB was also used as an osmoprotectant. Dimethylglycine oxidase (DMGO) was involved in the catabolism of GB. Two genes for DMGO were detected in a cloned 26267 bp fragment of A. globiformis DNA. The genes involved in the tetrahydrofolate-dependent assimilation of methyl groups were located nearby the two of DMGO genes. Both cloned A. globiformis DMGO were active. The activity of DMGO was detected in A. globiformis cells and it depended on the addition of GB and the salinity of the medium. Reverse transcription-PCR demonstrated that the addition of GB influenced the transcription of dmg genes.
甘氨酸甜菜碱（GB）可以被球形节杆菌细胞用作唯一的碳源。细胞在低盐和高盐培养基中都能吸收这种分子。向高盐浓度的矿物培养基中添加 GB 表明，GB 也可用作渗透压保护剂。二甲基甘氨酸氧化酶（DMGO）参与 GB 的分解代谢。在球形节杆菌 DNA 的 26267bp 克隆片段中检测到两个 DMGO 基因。涉及四氢叶酸依赖性甲基同化的基因位于两个 DMGO 基因附近。克隆的两种球形节杆菌 DMGO 均具有活性。在球形节杆菌细胞中检测到 DMGO 的活性，并且其活性取决于 GB 的添加和培养基的盐度。逆转录-PCR 表明，GB 的添加影响 dmg 基因的转录。
