铜绿假单胞菌的甘氨酸甜菜碱转甲基酶突变体
Glycine betaine transmethylase mutant of Pseudomonas aeruginosa.
作者信息
Serra Ana L, Mariscotti Javier F, Barra José L, Lucchesi Gloria I, Domenech Carlos E, Lisa Angela T
机构信息
Departmento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, 5.800 Río Cuarto, Argentina.
出版信息
J Bacteriol. 2002 Aug;184(15):4301-3. doi: 10.1128/JB.184.15.4301-4303.2002.
Abstract
The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.
摘要
通过生化、生理和分子方法，在铜绿假单胞菌菲尔德斯三号菌株中鉴定出了甘氨酸甜菜碱转甲基酶（gbt）基因。基于序列分析，敲除基因对应于铜绿假单胞菌PAO1基因组中一个名为PA3082的开放阅读框（ORF）。该ORF的翻译产物与不同微生物的转移酶具有相似性。gbt基因的突变阻断了胆碱和甘氨酸甜菜碱作为碳源和氮源的利用。
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本文引用的文献
1
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.铜绿假单胞菌PAO1（一种机会致病菌）的全基因组序列
Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.
2
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
3
pBBR1MCS: a broad-host-range cloning vector.pBBR1MCS：一种广宿主范围的克隆载体。
Biotechniques. 1994 May;16(5):800-2.
4
Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons.利用源自Tn5和Tn10的微型转座子分析和构建革兰氏阴性菌的稳定表型
Methods Enzymol. 1994;235:386-405. doi: 10.1016/0076-6879(94)35157-0.
5
Carnitine resembles choline in the induction of cholinesterase, acid phosphatase, and phospholipase C and in its action as an osmoprotectant in Pseudomonas aeruginosa.肉碱在诱导胆碱酯酶、酸性磷酸酶和磷脂酶C方面以及在作为铜绿假单胞菌的渗透保护剂的作用方面与胆碱相似。
Curr Microbiol. 1995 Jan;30(1):55-60. doi: 10.1007/BF00294525.
6
Induction of acid phosphatase and cholinesterase activities in Ps. aeruginosa and their in-vitro control by choline, acetylcholine and betaine.铜绿假单胞菌中酸性磷酸酶和胆碱酯酶活性的诱导及其在体外受胆碱、乙酰胆碱和甜菜碱的控制。
Mol Cell Biochem. 1983;50(2):149-55. doi: 10.1007/BF00285640.
7
Trans-sulfuration in mammals. The methionine-sparing effect of cystine.哺乳动物中的转硫作用。胱氨酸的蛋氨酸节约效应。
J Biol Chem. 1967 Mar 10;242(5):873-80.
8
Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti.苜蓿根瘤菌中甘氨酸甜菜碱生物合成与降解的渗透调控
J Bacteriol. 1988 Jul;170(7):3142-9. doi: 10.1128/jb.170.7.3142-3149.1988.
9
Transposon insertion mutagenesis of Pseudomonas aeruginosa with a Tn5 derivative: application to physical mapping of the arc gene cluster.用Tn5衍生物对铜绿假单胞菌进行转座子插入诱变：应用于arc基因簇的物理图谱绘制。
Gene. 1985;33(3):293-303. doi: 10.1016/0378-1119(85)90237-9.
10
Codon usage in Pseudomonas aeruginosa.铜绿假单胞菌中的密码子使用情况。
Nucleic Acids Res. 1988 Oct 11;16(19):9323-35. doi: 10.1093/nar/16.19.9323.
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