Guo Zhanlin, Zhou Qinghua, Zhu Wen, Li Dairong, Yuan Tianzhu, Wang Yanping, Chen Xiaohe, Liu Lunxu, Wu Zhu, Yang Junjie
Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province, Department of Thoracocardiac Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Zhongguo Fei Ai Za Zhi. 2005 Apr 20;8(2):89-94. doi: 10.3779/j.issn.1009-3419.2005.02.03.
Genetic polymorphism in metabolic enzymes, which are involved in metabolism of environmental carcinogens, have been thought to be related to susceptibility of lung cancer. The aim of this study is to investigate the cytochrome P450 2D6(CYP2D6) genetic polymorphism distribution in Han population in Sichuan, China, and to evaluate the relationship between CYP2D6 genetic polymorphism and lung cancer susceptibility.
PCR-RFLP was used to identify CYP2D6ch genotypes among 150 patients with primary lung cancer and 152 healthy controls, in Han population in Sichuan, China, and case-control study was used to analyze the relationship between genetic polymorphism and lung cancer susceptibility.
(1) The distribution frequency of CYP2D6ch C and T allele were 39.5% and 60.5% in control group and 46.3% and 53.7% in lung cancer group, respectively. There was no significant difference between the two groups (P=0.089). (2)The distribution frequency of C/C, C/T and T/T genotypes were 18.4%, 42.1% and 39.5% in control group, and 22.7%, 47.3% and 30.0% in lung cancer group, respectively. No significant difference was found between the two groups (P=0.215). (3) The individuals who carried with Non-T/T genotypes had a 2.084-fold increased risk with squamous cell carcinoma (95%CI 1.024-4.244, P=0.043) than those who carried with T/T genotype. (4) The lighter smokers ( < 30 pack-years) who carried with Non-T/T genotypes had a 2.92-fold increased risk with lung cancer (95%CI 1.087-7.828, P=0.033) than those who carried with T/T genotype.
CYP2D6ch Non-T/T genotypes are factors associated mail:zhouqh@mail.sc.cninfo.net) with increased risk of squamous cell carcinoma and also increase risk of lung cancer among lighter smokers.
参与环境致癌物代谢的代谢酶基因多态性被认为与肺癌易感性有关。本研究旨在调查中国四川汉族人群中细胞色素P450 2D6（CYP2D6）基因多态性分布，并评估CYP2D6基因多态性与肺癌易感性之间的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对150例原发性肺癌患者和152例健康对照者（中国四川汉族人群）进行CYP2D6ch基因型鉴定，并采用病例对照研究分析基因多态性与肺癌易感性之间的关系。
（1）对照组中CYP2D6ch C和T等位基因的分布频率分别为39.5%和60.5%，肺癌组分别为46.3%和53.7%。两组间差异无统计学意义（P=0.089）。（2）对照组中C/C、C/T和T/T基因型的分布频率分别为18.4%、42.1%和39.5%，肺癌组分别为22.7%、47.3%和30.0%。两组间差异无统计学意义（P=0.215）。（3）携带非T/T基因型的个体患鳞状细胞癌的风险比携带T/T基因型的个体高2.084倍（95%可信区间1.024-4.244，P=0.043）。（4）吸烟量较轻（<30包年）且携带非T/T基因型的个体患肺癌的风险比携带T/T基因型的个体高2.92倍（95%可信区间1.087-7.828，P=0.033）。
CYP2D6ch非T/T基因型是与鳞状细胞癌风险增加相关的因素，也是吸烟量较轻者患肺癌风险增加的因素。 邮箱：zhouqh@mail.sc.cninfo.net
