Yuan Tianzhu, Zhou Qinghua, Zhu Wen, Guo Zhanlin, Li Dairong, Wang Yanping, Chen Xiaohe, Liu Lunxu, Yang Junjie
Key Laboratory of Lung Cancer Molecular Biology of Sichuan Province, Department of Thoracocardiac Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Zhongguo Fei Ai Za Zhi. 2005 Apr 20;8(2):107-11. doi: 10.3779/j.issn.1009-3419.2005.02.07.
Some studies have supposed that glutathione S-transferases (GSTs) may be involved in detoxification of carcinogens, especially from tobacco smoke. Therefore, polymorphism of GSTs has been considered as potential protectors of individual cancer risk. The objective of this study is to investigate the relationship between genetic polymorphism of GSTT1 and inherent susceptibility to lung cancer in Han population in Sichuan, China.
A case-control study was carried out to compare the distribution frequency of GSTT1 gene polymorphism between lung cancer (n=150) and control healthy individuals (n=152) with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and to analyze the relationship between the GSTT1 gene polymorphism and smoking and the inherent susceptibility of lung cancer.
(1) The distribution frequency of GSTT1(-) genotype was 54.7% (82/150) in lung cancer and 38.2% (58/152) in control group respectively (OR=1.681, 95%CI=1.009- 2.803 , P=0.046); (2) GSTT1(-) genotype remarkably increased the risk of squamous cell carcinoma (OR=2.969, 95%CI= 1.511 -5.834, P=0.002) and adenocarcinoma (OR=2.095, 95%CI=1.060-4.140, P= 0.033 ); (3) In smokers, GSTT1(-) genotype significantly increased the risk for lung cancer (OR=4.051, 95%CI=1.959-8.380 , P=0.000); (4) In people with GSTT1(-) genotype, smoking markedly increased the risk for lung cancer (OR=53.885, 95%CI=11.789-246.302, P=0.000); (5) In heavy smokers (≥20 packyears), GSTT1(-) genotype could remarkably increase the risk of lung cancer (OR=4.296, 95%CI=1.649-11.190, P=0.003).
(1) People with GSTT1(-) genotype have significantly increased risk for lung cancer in Han population in Sichuan, China, especially for squamous cell carcinoma. (2) GSTT1(-) genotype interacts synergistically with smoking on lung cancer risk. The more the cigarettes smoke, the higher the risk of lung cancer increases in those people who are smokers with GSTT1(-) genotype.
一些研究认为谷胱甘肽S-转移酶（GSTs）可能参与致癌物的解毒过程，尤其是来自烟草烟雾中的致癌物。因此，GSTs的基因多态性被认为是个体癌症风险的潜在保护因素。本研究的目的是探讨中国四川汉族人群中GSTT1基因多态性与肺癌内在易感性之间的关系。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行病例对照研究，比较肺癌患者（n=150）和健康对照个体（n=152）中GSTT1基因多态性的分布频率，并分析GSTT1基因多态性与吸烟及肺癌内在易感性之间的关系。
（1）肺癌组中GSTT1（-）基因型的分布频率为54.7%（82/150），对照组为38.2%（58/152）（OR=1.681，95%CI=1.009-2.803，P=0.046）；（2）GSTT1（-）基因型显著增加了鳞状细胞癌（OR=2.969，95%CI=1.511-5.834，P=0.002）和腺癌（OR=2.095，95%CI=1.060-4.140，P=0.033）的发病风险；（3）在吸烟者中，GSTT1（-）基因型显著增加了患肺癌的风险（OR=4.051，95%CI=1.959-8.380，P=0.000）；（4）在GSTT1（-）基因型人群中，吸烟显著增加了患肺癌的风险（OR=53.885，95%CI=11.789-246.302，P=0.000）；（5）在重度吸烟者（≥20包年）中，GSTT1（-）基因型可显著增加患肺癌的风险（OR=4.296，95%CI=1.649-11.190，P=0.003）。
（1）在中国四川汉族人群中，GSTT1（-）基因型个体患肺癌的风险显著增加，尤其是鳞状细胞癌。（2）GSTT1（-）基因型与吸烟在肺癌风险上存在协同作用。对于携带GSTT1（-）基因型的吸烟者，吸烟量越大，患肺癌的风险越高。
