Gaspar A, Edlinger E
Ann Microbiol (Paris). 1978 May-Jun;129(4):545-52.
Electrosyneresis (counter-current immunoelectrophoresis) detected the presence of 7 coxsackieviruses and 1 poliovirus in 70 specimens of stools. Identical results were obtained by inoculation of mice and tissue cultures. First screening of specimens was undertaken with a human "standard" gammaglobulin; only specimens precipitating with this reagent contained viral antigens. Hence, especially in children, all negative stools could be excluded from subsequent tests with antiserum pools and monospecific antisera. In our experience, electrosyneresis is a specific method: every type of coxsackievirus and poliovirus types react only with the corresponding monospecific antisera. Electrosyneresis appears to be a sensitive procedure: viral antigens corresponding to 10(4) TCID50/ml can be dtected.
电渗析（逆流免疫电泳）在70份粪便标本中检测到7种柯萨奇病毒和1种脊髓灰质炎病毒。通过接种小鼠和组织培养获得了相同的结果。首先用人“标准”丙种球蛋白对标本进行筛查；只有与该试剂沉淀的标本才含有病毒抗原。因此，特别是在儿童中，所有阴性粪便可被排除在随后使用抗血清池和单特异性抗血清的检测之外。根据我们的经验，电渗析是一种特异性方法：每种柯萨奇病毒和脊髓灰质炎病毒型仅与相应的单特异性抗血清发生反应。电渗析似乎是一种灵敏的方法：可以检测到相当于10(4) TCID50/ml的病毒抗原。
