Bell E J, Cosgrove B P
Bull World Health Organ. 1980;58(3):423-8.
For many years a substitute cell line has been sought to replace monkey kidney cell cultures for the diagnosis of enterovirus infections. Reports by various workers have shown that the RD cell line, derived from a human rhabdomyosarcoma, will support the replication of most of the prototype strains of enterovirus. The present study shows that, with the exception of the group B coxsackieviruses, RD cells are more sensitive than cynomolgus monkey kidney cultures for the isolation of a wide variety of enteroviruses from clinical specimens. Since regular access by many diagnostic laboratories to supplies of primary cell cultures is often difficult because of distance from source or cost factors, a simple cell culture system is proposed which should prove useful for the diagnosis of most of the important enterovirus infections.
多年来，人们一直在寻找一种替代细胞系，以取代猴肾细胞培养物用于肠道病毒感染的诊断。不同研究人员的报告表明，源自人横纹肌肉瘤的RD细胞系能够支持大多数肠道病毒原型株的复制。本研究表明，除了B组柯萨奇病毒外，RD细胞在从临床标本中分离多种肠道病毒方面比食蟹猴肾培养物更敏感。由于许多诊断实验室常常因距离来源地远或成本因素而难以定期获得原代细胞培养物供应，因此提出了一种简单的细胞培养系统，该系统应被证明对大多数重要肠道病毒感染的诊断有用。
