蛋白质免疫印迹法：从样品制备到检测
Western blotting: sample preparation to detection.
作者信息
Eslami Anna, Lujan Jesse
机构信息
Research and Development, EMD Chemicals Inc.
出版信息
J Vis Exp. 2010 Oct 14(44):2359. doi: 10.3791/2359.
Abstract
Western blotting is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
摘要
蛋白质免疫印迹法是一种分析技术，用于检测给定组织匀浆或提取物样本中的特定蛋白质。它利用凝胶电泳，根据多肽的长度（变性条件下）或蛋白质的三维结构（天然/非变性条件下）来分离天然或变性蛋白质。然后将蛋白质转移到一张膜上（通常是硝酸纤维素膜或聚偏二氟乙烯膜），在膜上使用针对目标蛋白质的特异性抗体进行探测（检测）。
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