Amati B, Gasser S M
Swiss Institute for Experimental Cancer Research (ISREC), Epalinges s/Lausanne.
Mol Cell Biol. 1990 Oct;10(10):5442-54. doi: 10.1128/mcb.10.10.5442-5454.1990.
Histone-depleted nuclei maintain sequence-specific interactions with genomic DNA at sites known as scaffold attachment regions (SARs) or matrix attachment regions. We have previously shown that in Saccharomyces cerevisiae, autonomously replicating sequence elements bind the nuclear scaffold. Here, we extend these observations to the fission yeast Schizosaccharomyces pombe. In addition, we show that four SARs previously mapped in the genomic DNA of Drosophila melanogaster bind in vitro to nuclear scaffolds from both yeast species. In view of these results, we have assayed the ability of the Drosophila SARs to promote autonomous replication of plasmids in the two yeast species. Two of the Drosophila SARs have autonomously replicating sequence activity in budding yeast, and three function in fission yeast, while four flanking non-SAR sequences are totally inactive in both.
组蛋白缺失的细胞核在被称为支架附着区域（SARs）或基质附着区域的位点与基因组DNA保持序列特异性相互作用。我们之前已经表明，在酿酒酵母中，自主复制序列元件与核支架结合。在这里，我们将这些观察结果扩展到裂殖酵母粟酒裂殖酵母。此外，我们表明，先前在黑腹果蝇基因组DNA中定位的四个SARs在体外与两种酵母的核支架结合。鉴于这些结果，我们检测了果蝇SARs在两种酵母中促进质粒自主复制的能力。两个果蝇SARs在芽殖酵母中具有自主复制序列活性，三个在裂殖酵母中起作用，而四个侧翼非SAR序列在两者中完全无活性。
