Johnston L H, Barker D G
Mol Gen Genet. 1987 Apr;207(1):161-4. doi: 10.1007/BF00331504.
A DNA sequence has been isolated from Schizosaccharomyces pombe which promotes high frequency transformation of plasmids in the same organism. It is closely linked to the DNA ligase gene CDC17 and has therefore been named ARS17 although in structure it differs substantially from ARS elements in Saccharomyces cerevisiae. ARS17 spans some 1.8 kb of DNA and deletion of any part of this region affects activity. Moreover, there does not appear to be any short sequence which is, by itself, sufficient for high frequency transformation. ARS17 lies between and partly overlaps two divergently transcribed genes and it is extremely AT rich. It lacks the consensus sequence found in S. cerevisiae ARSs and it has no ARS activity in S. cerevisiae.
从粟酒裂殖酵母中分离出一段DNA序列，该序列可促进同一生物体中质粒的高频转化。它与DNA连接酶基因CDC17紧密相连，因此被命名为ARS17，尽管其结构与酿酒酵母中的ARS元件有很大不同。ARS17跨越约1.8 kb的DNA，该区域任何部分的缺失都会影响活性。此外，似乎不存在任何单独足以实现高频转化的短序列。ARS17位于两个反向转录的基因之间并部分重叠，且富含AT。它缺乏酿酒酵母ARS中发现的共有序列，在酿酒酵母中也没有ARS活性。
